| Description | The antibody 47D1 is a human mAb specific for SARS-CoV-2 Spike S1 protein. The antibody binds only to one side of the receptor binding surface on the RBD. Neutralization by the antibody is achieved independent of interfering RBD-ACE2 binding. A crystal structure of the mAb-RBD complex shows that the IF motif at the tip of the antibody CDR H2 interacts with a hydrophobic pocket in the RBD. It neutralizes SARS-CoV-2 by suppressing spike-mediated membrane fusion, rather than blocking virus binding to ACE2-expressing cells. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | Receptor-binding domain (RBD) |
| Function | Suppresses spike-mediated membrane fusion, but not blocking the virus binding to ACE2-expressing cells. |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA, Neut |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Western Blot Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
