| Description | This antibody is a human neutralizing antibody clone SNV-42 recognizing the SNV Gn (Sin Nombre virus surface glycoprotein). It interferes with both receptor recognition and fusion during host-cell entry. It shows exceptionally potent neutralizing activity for SNV in vitro. This product has been tested using Enzyme-linked immunosorbent assay, Bio-layer interferometry and Neutralization assay. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Sin Nombre virus (SNV) |
| Epitope | SNV-42 binds to domain B and the E3-like domain of SNV Gn. |
| Neutralization Mechanism | SNV-42 disrupts recognition of sEC1-EC2 and mediates neutralizing activity after the virus particle attaches to cells. |
| IC50 | 21.4 ng/mL |
| Affinity | 1.0 pM |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; BLI; Neut |
| Application Notes | Bio-layer interferometry (BLI): Antibody was allowed to associate with SNV GnH for 300 s in a serial dilution scheme starting at 200 nM at a 1:2 dilution. Neutralization assay (Neut): VSV/SNV were mixed with mAb in duplicate in a volume of 100 µl using DMEM and incubated for 1 h at 37°C. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SNV Gn |
| Alternative Name | Sin Nombre virus surface glycoprotein |
| Research Area | Infectious Disease |
| Related Disease | Severe respiratory failure |
