Description | This product is a recombinant human antibody provided by CreativeBiolabs. The antibody is specific for Transforming Growth Factor Beta Receptor 2, the protein encoded by this gene is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with TGF-beta receptor type-1, and binds TGF-beta. It can be used for TGFBR2 detection in Enzyme-Linked Immunosorbent Assay. It was expressed in mammalian cells (293F or CHO) with antibody encoding genes and purified by affinity chromatography. Each lot of this antibody is quality control tested by SDS-PAGE and SEC-HPLC analysis. For highly sensitive assays, we recommend the ultrapurified form of the product, which has a lower endotoxin limit than standard antibody, less than 1 EU/mg or even 0.1 EU/mg. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | Human |
Isotype | IgG |
Expression Species | HEK293F or CHO Cell line |
Conjugation | Unconjugated, also available for Biotin, HRP, FITC and PE-labeled form. |
Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
Endotoxin | <1 EU/mg, determined by LAL method |
Purification | Purified with Protein A or G affinity chromatography |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
Application | ELISA |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | TGFBR2 |
Alternative Name | Transforming Growth Factor Beta Receptor 2; Transforming Growth Factor, Beta Receptor II (70/80kDa); Transforming Growth Factor Beta Receptor II; TGF-Beta Type II Receptor; EC 2.7.11.30; TbetaR-II; TGFR-2; Transforming Growth Factor, Beta Receptor II Epsilon; Transforming Growth Factor, Beta Receptor II Alpha; Transforming Growth Factor, Beta Receptor II Delta; Transforming Growth Factor, Beta Receptor II Gamma; Transforming Growth Factor Beta Receptor Type IIC; Transforming Growth Factor, Beta Receptor II Beta; Transforming Growth Factor-Beta Receptor Type II; TGF-Beta Receptor Type IIB |
Gene ID | 7048 |
UniProt | P37173 |
Research Area | Cardiovascular |
Related Disease | Cancer |
Figure 1 Anti-TGFBR2 Recombinant Antibody (V3S-1022-YC89) in ELISA.
ELISA analysis of V3S-1022-YC89 was performed by coating with recombinant human TGFBR2 protein (ECD, His Tag).
The secondary antibody: HRP-anti-human IgG
Figure 1 Anti-TGFBR2 Recombinant Antibody (V3S-1022-YC89) in ELISA.
Figure 2 Anti-TGFBR2 Recombinant Antibody (V3S-1022-YC89) in Dot Blot.
Dot blot analysis of V3S-1022-YC89 was performed by coating with recombinant human TGFBR2 protein (ECD, His Tag).
The secondary antibody: HRP-anti-human IgG
Figure 2 Anti-TGFBR2 Recombinant Antibody (V3S-1022-YC89) in Dot Blot.