| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Transglutaminase 2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Immunogen | Recombinant protein containing the amino acid 143 to 473 of human TG2 core The sequence: CPADAVYLDSEEERQEYVLTQQGFIYQGSAKFIKNIPWNFGQFEDGILDICLILLDVNPKFLKNAGRDCSRRSSPVYVGRVVSGMVNCNDDQGVLLGRWDNNYGDGVSPMSWIGSVDILRRWKNHGCQRVKYGQCWVFAAVACTVLRCLGIPTRVVTNYNSAHDQNSNLLIEYFRNEFGEIQGDKSEMIWNFHCWVESWMTRPDLQPGYEGWQALDPTPQEKSEGTYCCGPVPVRAIKEGDLSTKYDAPFVFAEVNADVVDWIQQDDGSVHKSINRSLIVGLKISTKSVGRDEREDITHTYKYPEGSSEEREAFTRANHLNKLAEKEETGM. |
| Epitope | The antibody is to bind in whole or in part for a single epitope appears to be positioned in front of the active position covering the amino acid 304 to 326 and the substrate binding pocket. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; Inhib |
| Application Notes | The antibody is recommended for detection of TGM2 by ELISA, Inhib assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | TGM2 |
| Alternative Name | Transglutaminase 2; C Polypeptide, Protein-Glutamine-Gamma-Glutamyltransferase; Tissue Transglutaminase; Transglutaminase C; Transglutaminase H; EC 2.3.2.13; TGase C; TGase H |
| Gene ID | 7052 |
| UniProt | P21980 |
| Research Area | Cell Biology; Signal Pathway |
| Related Disease | Fibrosis-related disorder, Neurodegenerative/neurological disease and Cancer |
