| Sub Cat | Immunogen | Application | Affinity | |
|---|---|---|---|---|
| V3S-1124-YC607 | Tie1 extracellular domain | ELISA; FC; FuncS | 5.8 nM | |
| V3S-0522-YC687 | Purified recombinant human Tie-1-Fc target antigen | ELISA; FC; WB; IHC; IF; Agonist | 5.83 nM |
| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains 1. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Mouse |
| Immunogen | Tie1 extracellular domain |
| Epitope | Within the ECD domain. |
| Affinity | KD = 5.8 nM |
| Isotype | IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; FC; FuncS |
| Application Notes | The antibody is recommended for detection of TIE1 by ELISA, FC, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | TIE1 |
| Alternative Name | Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains 1; Tyrosine Kinase With Immunoglobulin And Epidermal Growth Factor Homology Domains 1; EC 2.7.10.1; TIE; Tyrosine Kinase With Immunoglobulin-Like And EGF-Like Domains 1; Tyrosine-Protein Kinase Receptor Tie-1; EC 2.7.10; JTK14; |
| Gene ID | 7075 |
| UniProt | P35590 |
| Research Area | Signal Pathway |
| Related Disease | Angiogenesis-associated disorders |
