| Description | This product is a monoclonal antibody derived from Human (Homo sapiens), which can specifically recognize Triggering receptor expressed on myeloid cells 2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Mouse |
| Immunogen | The extracellular domain of TREM2 |
| Epitope | Within the ECD domain. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; FC; WB; FuncS |
| Application Notes | The antibody is recommended for detection of TREM2 by ELISA, FC, WB, FuncS assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Anti-TREM2 Monoclonal Antibody (V3S-0522-YC1180) in SDS-PAGE
SDS-PAGE analysis of V3S-0522-YC1180 in non-reduced and β-mercaptoethanol-reduced conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
Figure 1 Anti-TREM2 Monoclonal Antibody (V3S-0522-YC1180) in SDS-PAGE
Figure 2 Anti-TREM2 Monoclonal Antibody (V3S-0522-YC1180) in SEC-HPLC
The purity of V3S-0522-YC1180 was greater than 97% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.3
Detection: UV 280 nm
Injection: 25 µl
Figure 2 Anti-TREM2 Monoclonal Antibody (V3S-0522-YC1180) in SEC-HPLC
Figure 3 Anti-TREM2 Monoclonal Antibody (V3S-0522-YC1180) in ELISA
ELISA analysis of V3S-0522-YC1180 was performed by coating with Recombinant Human TREM2 Protein.
Figure 3 Anti-TREM2 Monoclonal Antibody (V3S-0522-YC1180) in ELISA
