| Description | The mouse antibody TyTx4 recognizes the typhoid toxin. TyTx4 binds to PltB subunits and its epitope overlaps with glycan receptor-binding sites on PltB subunits. It neutralizes the increased dose of typhoid toxin effectively and it almost completely inhibits typhoid toxin binding to brain endothelial cells in vivo assay. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Salmonella typhi |
| Immunogen | Recombinant typhoid toxoid |
| Epitope | Its epitope overlaps with glycan receptor-binding sites on PltB subunits. |
| Neutralization Mechanism | TyTx4 inhibits typhoid toxin binding to cell surface glycan receptors and results in neutralization of typhoid toxin-mediated cellular toxicity. |
| Affinity | 0.028 nM |
| Isotype | Mouse IgG2a |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; WB; SPR; IF; FC; Neut |
| Application Notes | This antibody was tested using ELISA, Western Blot (WB), Surface plasmon resonance (SPR), Immunofluorescence (IF), Flow cytometry (FC) and Neutralization assay. Surface plasmon resonance (SPR): Pilot experiments to optimize experimental parameters were then performed using 10-fold serial dilutions of each MAb injected at a flow rate of 50 μL/min with a contact time of 120 sec and dissociation time of 600 sec. Immunofluorescence (IF): The slides were stained with indicated antibodies for 2 hrs at room temperature, counterstained with DAPI, mounted using ProLong antifade mounting solution, and viewed. Neutralization assay (Neut): Infected cells were then incubated in complete cell culture medium containing 10 μg/mL gentamicin for 72 hours in the presence and absence of 10 nM MAbs. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | Typhoid Toxin PltB |
| Research Area | Infectious Disease |
| Related Disease | Typhoid fever |
