| Sub Cat | Epitope | Isotype | Application | |
|---|---|---|---|---|
| V3S-1124-YC942 | IgG2a | ELISA; Neut | ||
| V3S-0522-YC350 | The epitope is E30, T34, T35, R44, N46, F47, K48, G49, G50, Y51, N59, E60, V62, L63, S64 and additional peptide 58 residues K98, K99, K100 and S102. | IgG2a | ELISA; Block | |
| V3S-0522-YC354 | The epitope is N9, D75, Y76, Q122, L124, D126, K163, T187, P188, and N190. | IgG2a | ELISA; Block | |
| V3S-0522-YC349 | The epitope is E30, T34, T35, R44, N46, F47, K48, G49, G50, Y51, N59, E60, V62, L63, S64 and additional peptide 58 residues K98, K99, K100 and S102. | IgG | ELISA; FuncS | |
| V3S-0522-YC345 | The epitope is residues 91-110. | IgG | WB; ELISA; IP; IF |
| Description | This product is a monoclonal antibody derived from Mouse (Mus musculus), which can specifically recognize Vaccinia virus envelope protein D8. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Vaccinia Virus (VACV) |
| Immunogen | VACV IMV membrane protein D8 |
| Isotype | IgG2a |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; Neut |
| Application Notes | The antibody is recommended for detection of VACV D8 by ELISA (0-10 μg/mL), Neut assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | VACV D8 |
| Alternative Name | D8; IMV membrane protein |
| UniProt | Q1M1K6 |
| Research Area | Microbiology; Infectious Disease |
