| Description | This product is a monoclonal antibody derived from Chicken (Gallus gallus), which can specifically recognize Capsid protein VP2. The antibody is expressed with mammalian cell transient expression system, serum-free and purified by affinity chromatography. The purity and integrity are tested via SDS-PAGE and SEC-HPLC analysis. Given an antigen, additional QC measures are also desired such as affinity testing and binding validation. Specifically, the antibody is provided in multiple formats for in vivo and in vitro assays. The Invivo version features greater than 95% purity, ultra-low endotoxin levels (<1 EU/mg or 0.1 EU/mg), and is preservative, stabilizer, and carrier protein-free. |
| Clonality | Monoclonal |
| Host Species | Chicken |
| Target Species | Very virulent infectious bursal disease virus (vvIBDV) |
| Immunogen | Very virulent infectious bursal disease virus (vvIBDV) strain CS89 |
| Epitope | Ile(256) and Ile(294) in the hypervariable region of VP2 may play roles in binding of CRAb 88. |
| Isotype | IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and/or SEC-HPLC |
| Endotoxin | <1 EU/mg, determined by LAL method |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4⁰C within a week. For longer storage, aliquot and store at -20⁰C. |
| Application | ELISA; IP; WB |
| Application Notes | The antibody is recommended for detection of vvIBDV VP2 by ELISA, IP, WB assays. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | vvIBDV VP2 |
| Alternative Name | VP2; Capsid protein VP2 |
| Research Area | Infectious Disease |
| Related Disease | vvIBDV infection |
