| Description | This product is a mouse monoclonal antibody that recognizes ZIKV E protein. It was isolated rapidly and efficiently from a ribosome-displayed antibody library constructed from the spleens of immunized mice. This product can be used in ELISA, WB, PRNT. It could bind specifically to ZIKV E protein tested by ELISA assays. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | ZIKV (Asian genotype (PRVABC59) |
| Immunogen | Isolated ribosome display of the antibody library generated from the spleens of five mice immunized with ZIKV. |
| Neutralization Potency | PRNT50=10.1 μg/mL |
| Affinity | KD =19 nM |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservative |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; PRNT |
| Application Notes | To determine binding affinity (an apparent Kd), the antibody was tested for binding to the protein ZIKV E (200 ng/well) at a concentration range of 200 nM to 0.01 nM. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
