| Description | This monoclonal antibody specifically targets the C. difficile toxin B. It has been observed that the antibody does not interact with the purified native toxin A. Through immunoblotting, it was determined that the mAb does interact with denatured toxin B. Furthermore, testing revealed that the purified antibody displays immunoreactivity, exhibiting a positive reaction to native toxin B with a binding affinity of 18 pM. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | C. difficile |
| Immunogen | C-terminal 250-amino-acid polypeptide of C. difficile toxin B. |
| Epitope | C-terminus |
| Affinity | KD = 18 pM |
| Isotype | Mouse IgG1, kappa |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | WB; ELISA |
| Application Notes | ELISA: 1 ng/mL |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | C. difficile ToxB |
| Alternative Name | Clostridioides difficile toxin B; toxB |
| Research Area | Infectious Disease |
| Related Disease | Clostridium difficile infection |
