| Description | This recombinant human antibody (clone AK2) is against human cyclin-dependent kinase 4 (CDK4). AK2 could specifically recognize and interacted with recombinant human CDK4 and endogenous CDK4 in tumor cells by WB, IF and Co-IP. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; IF; WB; Co-IP |
| Application Notes | The binding affinity of AK2 with CDK4 was determinated by noncompetitive ELISA (1 μg/mL, 2 μg/mL, 4 μg/mL, 8 μg/mL). The antibody is recommended for detection of CDK4 by Immunofluorescence assay, Western Blot and Co-immunoprecipitation assays in HeLa and MCF-7 cells. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
