| Description | This recombinant human antibody (clone R2.NS1 E8) specifically recognizes conformational epitopes on the β-ladder domain (aa 178-273) of Dengue virus (DENV) non-structural protein 1 (NS1). NS1 is a glycoprotein component of DENV associating with viral replication, infection and immune evasion. R2.NS1 E8 could react with four dengue serotypes (DENV1-4). |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Dengue virus (DENV) |
| Epitope | β-ladder domain (aa 178-273) of NS1 protein |
| Isotype | Human IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | WB; ELISA; FC |
| Application Notes | The binding activities of this mAb to NS1 were examined by ELISA, Western Blot, and Flow Cytometry. The results of ELISA showed that this antibody had no cross-reaction with ZIKV NS1. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | DENV NS1 |
| Alternative Name | Dengue virus non-structural protein 1 |
| Research Area | Microbiology; Infectious Disease |
| Related Disease | Dengue |
