| Description | This human anti-GNB5 recombinant antibody (clone H9) is able to recognize endogenous GNB5 protein (full-length). H9 has been tested for use in ELISA and Western Blotting. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Chicken, Mouse, Rabbit |
| EC50 | 300 nM |
| Affinity | KD = 300 nM (scFv) |
| Function | Enhances GTPase-activating protein (GAP) activity of regulator of G protein signaling (RGS) proteins, hence involved in the termination of the signaling initiated by the G protein coupled receptors (GPCRs) by accelerating the GTP hydrolysis on the G-alpha subunits, thereby promoting their inactivation (Probable). Increases RGS9 GTPase-activating protein (GAP) activity, hence contributes to the deactivation of G protein signaling initiated by D2 dopamine receptors (PubMed: 27677260). May play an important role in neuronal signaling, including in the parasympathetic, but not sympathetic, control of heart rate. |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB |
| Application Notes | The antibody has been tested for use in ELISA (1 μg/mL) and Western Blotting (1 μg/mL). The result of WB showed the limit of detection of the GNB5-MBP fusion protein by the scFv-Fc was 10 ng. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
