| Description | This antibody is specific for histone H4 Lys20 monomethylation (H4K20me1). The specificity of the H4K20me1 antibody in living cells was verified using yeast mutants and mammalian cells. This antibody can be used to monitor the oscillation of H4K20me1 levels during the cell cycle. The affinity of the 15F11 scFv form (250 nM) is lower than the corresponding IgG form. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Non-species dependent |
| Immunogen | Synthetic peptide conjugated to keyhole limpet hemocyanin |
| Affinity | 250 nM |
| Isotype | IgG1, kappa |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; IB; IF; ChIP |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | H4K20me1 |
| Alternative Name | H4K20me1 |
