| Description | This product is a human IgG1 antibody that recognizes human HLA-A*11:01. The affinity of antibody-IgG1-A*11:01 was determined to be 0.16 nM by enzyme-linked immunosorbent assay. 2E3 binds primarily to the HLA alpha chain and partially interacts with beta-2-microglobulin. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Cross Reactivity | 2E3 was found to react to A*01:01, A*11:01, A*25:01, A*26:01, A*34:01, A*34:02, A*36:01, A*43 :01, A*66:01, A*80:01 and B*73:01 respectively. |
| Epitope | HLA alpha chain and beta-2-microglobulin |
| Affinity | KD = 0.16 nM |
| Isotype | Human IgG1 |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; FC; FuncS |
| Application Notes | The KD of 2E3-IgG1-A*11:01 was determined to be 0.16 nM using enzyme-linked immunosorbent assay (ELISA). |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | HLA-A*11:01 |
| Alternative Name | MHC class I antigen; HLA-A |
| Research Area | Immunology |
