Description | This human anti-HPeV1 VP0 recombinant antibody (clone 55) recognizes PeV-A1-VP0 (Harris strain). This antibody has no cross-reactivity with enterovirus types and shows specificity of parechoviruses. This antibody can widly react with various PeV types. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | Human parechovirus 1 (PeV1) |
Immunogen | E. coli recombinant PeV-A1-VP0 protein |
Epitope | A low affinity epitope at location 140-ELPKVFWDHQDK-151, and a high affinity epitope at position 192-VVTYDSKL-199. |
Isotype | Human IgG |
Expression Species | HEK293F or CHO cell line |
Conjugation | Unconjugated |
Purity | >95% |
Endotoxin | <1 EU/mg |
Form | Liquid |
Purification | Protein A purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
Application | ELISA; WB; IF |
Application Notes | The binding of the mAb to PeV-A1 (Harris strain) and VP0-GST was confirmed by sandwich ELISA, Western Blotting and Immunofluorescence assay. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | HPeV1 VP0 |
Alternative Name | VP0 |
Research Area | Microbiology |
Related Disease | Viral sepsis-like illness and meningitis (infants) |