| Description | This recombinant human antibody (clone 4D5-1) recognizes surface-expressed immunoglobulin (sIg) on human MEC1 cells. This antibody was generated by phage display and selection. This antibody has been tested in use for ELISA. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | Human IgG1, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA |
| Application Notes | The antibody is recommended for detection of NPCs by ELISA. The binding activites of phage 4D5-1 were characterized by Flow Cytometry on MEC1 cells. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | Human sIg |
| Alternative Name | Surface immunoglobulin; Human surface immunoglobulin |
| Research Area | Cell Biology; Immunology |
| Related Disease | B-cell malignancies |
