| Description | This HuG6.3 product is a recombinant human antibody,which recognizes human IGHV1-69. The humanized antibody HuG6.3 exhibited approximately a 2-fold increase in binding affinity for G6-id(+) antibodies compared to the original MuG6. HuG6.3 was capable of killing cells expressing G6-id(+) BCR and patient B-CLL cells through antibody-dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). Furthermore, both MuG6 and HuG6.3 were found to facilitate the in vivo depletion of B-CLL cells in NSG mice. These findings suggest that HuG6.3 may offer a novel precision medicine approach for selectively eliminating IGHV1-69-encoding G6-id(+) B-CLL cells. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA; ADCC; CDC; FuncS |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | IGHV1-69 |
| Alternative Name | IGHV1-69; Immunoglobulin Heavy Variable 1-69; IGHV1-E; IGHV1E; Immunoglobulin Heavy Variable 1-E |
