Description | This product is a human monoclonal antibody that recognizes ISG and can be used in ELISA. It was selected from the yeast-displayed human non-immune library. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | T. brucei |
Immunogen | Isolated from human non-immune yeast-display library and recombinant ISG75 from T. brucei gambiense LiTat1.3 as an antigen. |
Epitope | ISG75 |
Affinity | 52 nM, as determined by flow cytometric assay |
Isotype | Human IgG |
Expression Species | HEK293F or CHO cell line |
Conjugation | Unconjugated |
Purity | >95% |
Endotoxin | <1 EU/mg |
Form | Liquid |
Purification | Protein A purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservative |
Stabilizer | No stabilizers |
Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
Application | ELISA; SPR; FC; CIFC-Ag Assay; CIFC-Ab Assay |
Application Notes | CIFC-Ag assay was assembled that utilized yeast-displayed scFv, which binds specifically to ISG75 with an affinity of 52 nM. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | ISG75 |
Alternative Name | Invariant surface glycoproteins |
Research Area | Cancer Research |
Related Disease | HAT |