| Description | This product is a human monoclonal antibody that recognizes ISG and can be used in ELISA. It was selected from the yeast-displayed human non-immune library. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | T. brucei |
| Immunogen | Isolated from human non-immune yeast-display library and recombinant ISG75 from T. brucei gambiense LiTat1.3 as an antigen. |
| Epitope | ISG75 |
| Affinity | 52 nM, as determined by flow cytometric assay |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservative |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; SPR; FC; CIFC-Ag Assay; CIFC-Ab Assay |
| Application Notes | CIFC-Ag assay was assembled that utilized yeast-displayed scFv W75C6, which binds specifically to ISG75 with an affinity of 52 nM. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | ISG75 |
| Alternative Name | Invariant surface glycoproteins |
| Research Area | Cancer Research |
| Related Disease | HAT |
