| Description | This product is a sheep monoclonal antibody that recognizes M. avium subsp. paratuberculosis Surface Protein. The antibody SurfS1.2 and SurfS2.2 can be used in several different applications, like immunoblotting, flow cytometry, and immunomagnetic separation of the M. avium subsp. paratuberculosis pathogen from mixed cultures and fecal samples. |
| Clonality | Monoclonal |
| Host Species | Sheep |
| Target Species | M. avium subsp. |
| Immunogen | Recombinant M. avium subsp. paratuberculosis antigens |
| Epitope | Mycobacterium avium subsp. paratuberculosis Surface Proteins |
| Isotype | Sheep IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservative |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; FC; Immunomagnetic separation; FuncS |
| Application Notes | Immunoblotting of M. avium subsp. paratuberculosis lysate and detection with SurfS1.2. Flow cytometry to detect Mycobacterium sp. surface antigen recognition by clones SurfS1.2. SurfS1.2 (5 μg/ml)can influence mycobacterial growth of M. avium subsp. avium. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | M. avium subsp. paratuberculosis Surface Protein |
| Alternative Name | MPARA |
| Research Area | Microbiology |
| Related Disease | Johne's disease |
