| Description | This product is an inhibitory antibody that recognizes Pseudomonas aeruginosa SpuE. The scFv5 antibody greatly enhanced the survival of the nematode Caenorhabditis elegans when faced with P. aeruginosa infection. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Pseudomonas aeruginosa |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Inhib; FuncS |
| Application Notes | The antibody showed high apparent affinities in both direct binding ELISA (EC₅₀ = 1.6 nM) and competition ELISA (IC₅₀ = 16.7 nM). |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | P. aeruginosa SpuE |
| Alternative Name | Pseudomonas aeruginosa SpuE; type III secretion system; Pseudomonas aeruginosa; SpuE; P. aeruginosa |
| Research Area | Microbiology |