PCSK9 Specific Recomb™ Antibody (V3S-0923-CJ16), Human IgG (CAT#: V3S-0923-CJ16)

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Datasheet

MSDS

COA

Summary
Property
Applications
Protocols
Target
Virus Background

Summary

Description This recombinant anti-PCSK9 antibody (clone 3D2) recognizes human proprotein convertase subtilisin/kexin type 9. The PCSK9/LDLR pathway of 3D2-treated HepG2 cells was inhibited in vitro. 3D2 is an potential PCSK9 inhibitor and 3D2 inhibits PCSK9 function in vitro and in vivo.
Clonality Monoclonal
Host Species Human
Target Species Human
Affinity KD = 0.196±0.156 nM
Function Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments (PubMed: 18039658).
Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or direct it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation (PubMed: 18799458, PubMed: 17461796, PubMed: 18197702, PubMed: 22074827).
Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway (PubMed: 18660751).
Inhibits epithelial Na+ channel (ENaC)-mediated Na+ absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways.
Isotype Human IgG

Property

Expression Species HEK293F or CHO cell line
Conjugation Unconjugated
Purity >95%
Endotoxin <1 EU/mg
Form Liquid
Purification Protein A purified
Sterility 0.2 μM filtered
Formulation PBS, pH 7.4
Preservation No preservatives
Stabilizer No stabilizers
Storage Store at 4°C within a week. For longer storage, aliquot and store at -20°C.

Applications

Application ELISA; WB; Inhib
Application Notes The binding of the antibody to PCSK9 protein was detected by indirect ELISA. The levels of LDLR protein of mAb- and/or mevinolin-treated HepG2 cells were assessed by Western Blotting (antibody concentration: 5 µg/mL, 10 µg/mL). The antibody inhibited the PCSK9/LDLR pathway of Hep-G2 cells and resulted in a 3-fold increase in hepatic LDLR levels.

Protocols

ELISA Enzyme-Linked Immunosorbent Assay Protocol
WB Western Blot Protocol
FC Flow Cytometry Protocol

Target

Target PCSK9
Alternative Name FH3; PC9; FHCL3; NARC1; LDLCQ1; NARC-1; HCHOLA3
Gene ID 255738
UniProt Q8NBP7
Research Area Signal Pathway
Related Disease Cardiovascular disease; Hypercholesterolemia

Virus Background

Post Translational Modification Cleavage by furin and PCSK5 generates a truncated inactive protein that is unable to induce LDLR degradation.
Undergoes autocatalytic cleavage in the endoplasmic reticulum to release the propeptide from the N-terminus and the cleavage of the propeptide is strictly required for its maturation and activation. The cleaved propeptide however remains associated with the catalytic domain through non-covalent interactions, preventing potential substrates from accessing its active site. As a result, it is secreted from cells as a propeptide-containing, enzymatically inactive protein.
Phosphorylation protects the propeptide against proteolysis.
For research use only, not directly for clinical use.
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