| Description | This is a mouse antibody which binds to PCSK9 with high affinity. It specifically targets the helical conformation of the acidic stretch. Ab6E2 binds to PCSK9 with a KD of 10.5 ± 1.2 nM. |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Affinity | KD = 10.5 ± 1.2 nM |
| Isotype | Mouse IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; IP; WB; SPR |
| Application Notes | This antibody recognizes the 16 kDa prodomain of recombinant PCSK9 but not the 60 kDa band containing the catalytic and C-terminal domains in Western blot. This antibody was also used to immunoprecipitate I from HepG2, IM-95m, and H2073 cells Secreted native PCSK9 and recombinant PCSK9. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | PCSK9 |
| Alternative Name | FH3; PC9; FHCL3; NARC1; LDLCQ1; NARC-1; HCHOLA3; proprotein convertase subtilisin/kexin type 9; convertase subtilisin/kexin type 9 preproprotein; neural apoptosis regulated convertase 1; subtilisin/kexin-like protease PC9 |
| Gene ID | 255738 |
| UniProt | Q8NBP7 |
| Research Area | Signal Pathway |
