| Description | This anti-PCSK9 antibody (hF3-9) reacts with human and monkey PCSK9 proteins. It does not recognize mouse PCSK9. The parental murine monoclonal antibody (mF3-9) was generated in BALB/c mice by immunization with recombinant human PCSK9 (aa 31-692) with His tag. Anti-PCSK9 antibody can effectively antagonize the inhibition of LDL uptake by PCSK9, but cannot block the combination between PCSK9 and its ligand LDL-R. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human, Monkey |
| Immunogen | Recombinant human PCSK9 (aa 31-692) with His tag |
| Epitope | PCSK9 ECD, aa 31-692 |
| Affinity | KD = 0.42 nM (Human PCSK9); KD = 1.27 nM (Monkey PCSK9) |
| Isotype | Chimeric (mouse/human) IgG2, κ |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; Inhib |
| Application Notes | The binding specificity of antibody hF3-9 to human, monkey and mouse PCSK9 was measured by ELISA. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | PCSK9 |
| Alternative Name | FH3; FHCL3; HCHOLA3; LDLCQ1; NARC-1; NARC1; PC9 |
| Gene ID | 255738 |
| UniProt | Q8NBP7 |
| Related pathway | Plasma lipoprotein assembly, remodeling, and clearance; Cholesterol metabolism |
| Research Area | Signal Pathway; Cardiovascular |
| Related Disease | Familial Hypercholesterolemia |
| Post Translational Modification | Cleavage by furin and PCSK5 generates a truncated inactive protein that is unable to induce LDLR degradation. Undergoes autocatalytic cleavage in the endoplasmic reticulum to release the propeptide from the N-terminus and the cleavage of the propeptide is strictly required for its maturation and activation. The cleaved propeptide however remains associated with the catalytic domain through non-covalent interactions, preventing potential substrates from accessing its active site. As a result, it is secreted from cells as a propeptide-containing, enzymatically inactive protein. Phosphorylation protects the propeptide against proteolysis. |
