| Description | This product (clone 81) recognizes PCV2 capsid protein. PCV2 capsid protein is encoded by PCV2 open reading frame 2 (ORF2). The antibody reactivity was characterized by ELISA, western blot, and SPR. clone 81 has the high reactivity to the PCV2 capsid. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Porcine circovirus type 2 (PCV2) |
| Affinity | KD = 57.2 nM (scFv) |
| Function | Self-assembles to form the virion icosahedral capsid with a T=1 symmetry. This very small capsid (17 - 22 nm in diameter) allows the virus to be very stable in the environment and resistant to some disinfectants, including detergents. Essential for the initial attachment to heparan sulfate moieties and chondroitin sulfate B of the host cell surface proteoglycans. After attachment, the virus is internalized in a clathrin-, caveolae- and dynamin-independent, actin and Rho-GTPase-mediated pathway and traffics to the nucleus. The capsid protein binds and transports the viral genome and Rep across the nuclear envelope. |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; SPR |
| Application Notes | The binding specificity of this antibody to PCV2 capsid was analyzed by ELISA and Western Blot analysis. The binding affinity was determined using Surface Plasmon Resonance. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
