| Description | This recombinant anti-PDCoV N antibody (clone N53) is against the porcine deltacoronavirus (PDCoV) N protein. The results of ELISA, Western Blot and IF showed that N53 had excellent specificity with the PDCoV-infected cells and PDCoV N proteins. |
| Clonality | Monoclonal |
| Host Species | Pig |
| Target Species | Porcine deltacoronavirus (PDCoV) |
| Immunogen | PDCoV |
| Epitope | aa 82-96, GELPPNDTPATTRVT |
| Function | Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed: 28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed: 28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed: 22658127, PubMed: 25034862, PubMed: 25399552). |
| Isotype | Pig IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; IF; DB |
| Application Notes | The binding specificity of this antibody to PDCoV N protein was analyzed by indirect ELISA, Western Blot and Immunofluorescence. The epitope on the PDCoV N protein was identified by Dot Blot. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | PDCoV N |
| Alternative Name | PDCoV; N; Porcine deltacoronavirus N protein |
| Research Area | Microbiology |
| Related Disease | Severe diarrhea and intestinal pathological damage in piglets |
