Description | This is an inhibitory monoclonal antibody that specifically recognizes Pfs25. It binds near the canonical site 1 epitope. This antibody has the highest affinity for recombinant Pfs25 within its respective epitope bin and showed>80% oocyst reduction in SMFA at 100 μg/mL. |
Clonality | Monoclonal |
Host Species | Human |
Target Species | Plasmodium falciparum |
Epitope | Near the canonical site 1 epitope. |
Affinity | KD = 4.6 nM |
Isotype | Human IgG |
Expression Species | HEK293F or CHO |
Conjugation | None |
Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
Endotoxin | <1 EU/mg |
Purification | Protein A purified |
Sterility | 0.2 μM filtered |
Formulation | PBS, pH 7.4 |
Preservation | No preservatives |
Stabilizer | No stabilizers |
Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
Application | ELISA; Inhib |
Application Notes | This antibody showed>80% reduction in oocyst density at 100 μg/mL. The antibody is more potent than any other identified anti-Pfs25 antibody (IC₈₀ = 16 μg/mL) and has much less potent recombinant Pfs25 binding affinity in the same low nanomolar range (KD = 4.6 nM) as multiple other mAbs. |
ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
WB | Western Blot Protocol |
FC | Flow Cytometry Protocol |
Target | Pfs25 |
Alternative Name | Plasmodium falciparum transmission; Plasmodium falciparum; P. falciparum |
Research Area | Immunology |