| Description | This product is a nano antibody of a specific targeting human pro-TGF beta 3/LTBP3 complex, which comprises complementarity determining regions CDR1-CDR3. It can specifically bind to a human pro-TGF beta 3/LTBP3 complex and does not specifically bind to a monomeric protein of pro-TGF beta 3 and a monomeric protein of LTBP3 and a complex pro-TGF beta 1/LTBP3 and pro-TGF beta 1/LTBP1. |
| Clonality | Monoclonal |
| Host Species | Llama |
| Target Species | Human |
| Isotype | Single domain antibody |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservative |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | FC; ELISA |
| Application Notes | The fluorescent nanobody-EGFP fusion antibody was used as a detection antibody, FACS buffer (1 XPBS; 1% v/v FBS) was used as a buffer, and the detection was performed by flow cytometry. The pro-TGF beta 3/LTBP3 protein is used as a coating antigen to coat an ELISA plate, the nano antibody (with a 6xHis tag) prepared in the embodiment 1 is used as a detection primary antibody, the anti-HIS-HRP of horseradish peroxidase labeled secondary antibody is used as a detection secondary antibody, and the TMB substrate is used as a color reagent, and the detection is carried out according to an ELISA detection method. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | pro-TGFβ3 / LTBP3 complex |
| Alternative Name | pro-TGFβ3/LTBP3 |
| Research Area | Developmental Biology; Immunology |
