| Description | This recombinant human antibody (clone MS473) is against S. aureus toxic shock syndrome toxin (TSST)-1. MS473 could inhibit TSST-1-induced mitogenesis and cytokine release in vitro. MS473 reacted with the peptides T1 and T2 involved in the binding of TSST-1 to the variable domain of the TCR β-chain (Vβ). MS473 showed no significant binding activity for the peptides M1 and M2 involved in the interaction between TSST-1 and MHC class II. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | S. aureus |
| Neutralization Mechanism | Neutralize the superantigenic effect of TSST-1, prevent TSST-1-induced mitogenicity and cytokine production. |
| IC50 | 1.5 µM |
| IC100 | 3.5 µM |
| Function | Responsible for the symptoms of toxic shock syndrome. |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB |
| Application Notes | The binding specificity of this antibody to S. aureus TSST-1 was examined by ELISA and Western Blot. The mAb showed a complete inhibitory activity on TSST-1-induced proliferation of PBMCs at concentrations more than 3.5 µM (IC100). |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | S. aureus TSST-1 |
| Alternative Name | Toxic shock syndrome toxin; TSST; TSST-1 |
| UniProt | P06886 |
| Research Area | Microbiology |
| Related Disease | Toxic shock syndrome |
