| Description | This product is a neutralizing antibody that binds to Severe Acute Respiratory Syndrome Coronavirus 2. S309 potently neutralizes SARS-CoV-2 and SARS-CoV pseudoviruses as well as authentic SARS-CoV-2, by engaging the receptor-binding domain of the S glycoprotein. S309 recognizes an epitope containing a glycan that is conserved within the Sarbecovirus subgenus, without competing with receptor attachment. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | RBD of the S glycoprotein |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA |
| Application Notes | The following proteins were coated on 96-well enzyme-linked immunosorbent assay plates at the following concentrations: SARS-CoV RBD at 1 μg/ml, SARS-CoV-2 RBD at 10 μg/ml, ectodomains of SARS-CoV, SARS-CoV-2, HCoV-OC43 and MERS-CoV, all at 1 μg/ml. After blocking with 1% BSA in PBS, antibodies were added to the plates at concentrations between 5 and 0.000028 μg/ml and incubated for 1 h at room temperature. Plates were washed and secondary antibody Goat Anti Human IgG-AP was added. Substrate p-nitrophenyl pPhosphate (pNPP) was used for colour development. Optical density at 405 nm was read on an ELx808IU plate reader |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
