| Description | This product is a neutralizing antibody that binds to Severe Acute Respiratory Syndrome Coronavirus 2. S2H14 recognizes the RBD and S2H14 competed with ACE2 for recognition of the SARS-CoV-2 RBD indicating that S2H14 likely neutralizes SARS-CoV-2 through inhibition of virus/host cell interaction. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | SARS-CoV-2 |
| Epitope | RBD receptor binding domain |
| Isotype | IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein A purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within one or two weeks. Store at -20°C for long term. Avoid repeated freeze/thaw cycles. Refer to the COA file for specifics. |
| Application | ELISA |
| Application Notes | Spectraplate-384 with high protein binding treatment were coated overnight at 4°C with 1 μg/mL of SARS-CoV-2 S, domain A, N, S2, SARS-CoV S or 5 μg/mL of SARS-CoV-2/SARS-CoV RBD in PBS, pH 7.2, and plates were subsequently blocked with Blocker Casein (1%) in PBS supplemeted with 0.05% Tween 20. The coated plates were incubated with serial dilutions of human mAbs or human plasma or sera for 1 h at room temperature. The plates were then washed with PBS-T, and Alkaline Phosphatase-conjugated Goat Anti-Human IgG, IgM or IgA were added and incubated for 1 h. Plates were washed three times with PBS-T, and 4-NitroPhenyl Phosphate substrate was added and incubated for 1 h (IgG) or 2 h (IgA and IgM). The absorbance of 405 nm was measured by a microplate reader, and the data was plotted with Graph Prism software. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
| Target | SARS-CoV-2 |
| Alternative Name | Severe acute respiratory syndrome coronavirus 2 |
| Research Area | Coronavirus Disease 2019 |
| Related Disease | Coronavirus Disease 2019 |
