| Description | This recombinant human antibody (clone phage n.4) recognizes the product of the human erythrocyte membrane anion-transport protein gene (SLC4A1), erythroid band 3 protein. The DI blood group antigens (Dia and Dib) are located on the seventh extracellular loop of erythroid band 3 protein (aa 851-855). This antibody specifically binds Di(a+) but not Di(a-) red cells. |
| Clonality | Monoclonal |
| Host Species | Human |
| Target Species | Human |
| Epitope | The DI blood group antigens Dia (aa 851-855 on erythroid band 3 protein, which is the production of gene SLC4A1) |
| Isotype | Human IgG |
| Expression Species | HEK293F or CHO |
| Conjugation | None |
| Purity | >95%, determined by SDS-PAGE and SEC-HPLC |
| Endotoxin | <1 EU/mg |
| Purification | Protein A affinity purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservatives |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | FC; Inhib |
| Application Notes | The binding activities of this antibody with Di(a+) red cells were detected by Flow Cytometry. |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
