| Description | This product is a mouse monoclonal antibody that recognizes tau. The epitope of this antibody is phosphorylated tau (serine 202). |
| Clonality | Monoclonal |
| Host Species | Mouse |
| Target Species | Human |
| Epitope | phosphorylated serine 202 |
| Isotype | Mouse IgG1 |
| Expression Species | HEK293F or CHO cell line |
| Conjugation | Unconjugated |
| Purity | >95% |
| Endotoxin | <1 EU/mg |
| Form | Liquid |
| Purification | Protein G purified |
| Sterility | 0.2 μM filtered |
| Formulation | PBS, pH 7.4 |
| Preservation | No preservative |
| Stabilizer | No stabilizers |
| Storage | Store at 4°C within a week. For longer storage, aliquot and store at -20°C. |
| Application | ELISA; WB; IHC; IF |
| ELISA | Enzyme-Linked Immunosorbent Assay Protocol |
| WB | Western Blot Protocol |
| FC | Flow Cytometry Protocol |
Figure 1 Mouse Anti-phosphorylated Tau Recombinant Antibody (V3S-0723-WK89) in Western Blot.
Western blot analysis of V3S-0723-WK89 was performed by loading human pTau S202 protein-BSA.
V3S-0723-WK89 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-mouse IgG (H+L).
Figure 1 Mouse Anti-phosphorylated Tau Recombinant Antibody (V3S-0723-WK89) in Western Blot.
Figure 2 Mouse Anti-phosphorylated Tau Recombinant Antibody (V3S-0723-WK89) in Dot Blot.
Dot blot analysis of V3S-0723-WK89 was performed by coating with human pTau S202 protein-BSA.
V3S-0723-WK89 incubation concentration: 2 ng/μL.
HRP conjugated anti-mouse IgG as a secondary antibody (1: 2000).
Figure 2 Mouse Anti-phosphorylated Tau Recombinant Antibody (V3S-0723-WK89) in Dot Blot.
Figure 3 Mouse Anti-phosphorylated Tau Recombinant Antibody (V3S-0723-WK89) in ELISA.
ELISA analysis of V3S-0723-WK89 was performed by coating with human pTau S202 protein-BSA. Then blocked with BSA and incubated with V3S-0723-WK89. The HRP conjugated anti-mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Mouse Anti-phosphorylated Tau Recombinant Antibody (V3S-0723-WK89) in ELISA.
