Foot-and-Mouth Disease (FMD) is a highly contagious and economically one of the most important disease of transboundary importance. FMD is caused by infection with FMD virus (FMDV) belonging to the genus Aphthovirus of family Picornaviridae. FMDV has single stranded RNA genome of about 8000 bases size. The viral genome encodes 4 structural and 10 non-structural pro- teins (NSPs) through a single open reading frame. Individual viral proteins are further processed by the virus encoded proteases. Among all the NSPs of the FMDV, detection of antibodies directed against 3ABC region of the virus has been considered as the most reliable indicator of FMDV infection.