AAV Specific Neutra™ Antibody Products
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Are you currently facing challenges with viral vector tropism, neutralization, or the precise characterization of Adeno-associated virus (AAV) capsids? Creative Biolabs' AAV Specific Antibody Products help you overcome these hurdles and achieve reliable, reproducible results in your gene therapy and virology projects. We empower you to accelerate your research by providing high-quality, pre-validated antibodies for AAV detection and characterization.
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Introduction of AAV
Adeno-associated virus (AAV) is a compact, non-enveloped virus commonly employed as a gene delivery vector owing to its minimal immune reactivity. Its protein coat, or capsid, is assembled from VP1, VP2, and VP3 subunits, which play a key role in defining the virus's tropism—the capacity to selectively enter and transduce particular cell types. The complex architecture of the capsid and minor variations among VP proteins across serotypes contribute to AAV's versatility in diverse gene therapy uses. Each of the more than a dozen identified AAV serotypes (such as AAV1, AAV2, AAV5, AAV8, and AAV9) exhibits unique tissue targeting preferences, rendering them well-suited for transporting genetic payloads to designated tissues like the liver, muscle, or central nervous system. Despite being non-pathogenic, a major hurdle in gene therapy is that numerous people carry prior immunity to several AAV serotypes due to natural infection. This may result in vector clearance by the host's immune defenses, thereby diminishing treatment effectiveness. These factors underscore the necessity for accurate AAV profiling and neutralization antibody assessment prior to administering gene-based therapies.
Fig.1 Immune responses against AAVs.1
Our Anti-AAV Antibody Products
AAV antibodies are essential tools for gene therapy research and quality control, binding with high specificity to AAV capsid proteins for detection and quantification. Their applications include:
- Vector Titer and Quantitation: Used in immunoassays like ELISA to accurately quantify viral capsids for quality control.
- Tropism and In Vivo Studies: Used in immunohistochemistry to track AAV particles in different tissues.
- Neutralizing Antibody Assays: Used as reagents to detect pre-existing antibodies in patient serum, which is a crucial step for patient eligibility in clinical trials.
- Characterization of AAV Serotypes: Antibodies that distinguish between serotypes are vital for research and product development.
Creative Biolabs' highly characterized antibodies build upon this foundation, offering precise recognition for a range of applications.
Why Choose Us?
Creative Biolabs leads in antibody development with a commitment to scientific excellence and a deep understanding of biological targets. Our AAV Specific Antibody Products provide superior tools for gene therapy research.
Key advantages of working with us:
- Unparalleled Specificity: Our rigorously tested antibodies ensure precise AAV capsid binding, minimizing cross-reactivity.
- High Sensitivity: Our products detect AAV particles even at low concentrations, crucial for accurate quantitation.
- Reliable and Reproducible Results: We adhere to the highest scientific standards, ensuring consistent performance.
- Proven Expertise: Our team offers expert support to help you select the right products.
- Extensive Product Range: We provide a broad catalog of antibodies for various AAV serotypes.
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FAQs
Q: What is the primary use of these AAV-specific antibody products?
A: These products are primarily used for the detection, characterization, and quantification of AAV viral vectors. They are invaluable for quality control assays in gene therapy manufacturing, for studying the biodistribution of AAV vectors in preclinical models, and for developing assays to screen for pre-existing neutralizing antibodies in patient populations.
Q: How do I know which AAV serotype-specific antibody to choose for my project?
A: The choice of antibody depends on the AAV serotype you are using in your research. Our product line offers antibodies specific to a wide range of common serotypes, such as AAV1, AAV2, AAV5, AAV8, and AAV9. If you are unsure, our scientific support team can help you select the most suitable product for your specific application and AAV vector.
Q: Can these antibodies be used for in vivo applications or just for in vitro assays?
A: Our antibodies are primarily validated for in vitro applications such as ELISA, Western blotting, and immunofluorescence. While some researchers have successfully used similar antibodies for in vivo studies, such as blocking AAV transduction, this requires careful consideration of the specific antibody's characteristics and host organism. We always recommend contacting our team to discuss your specific in vivo needs.
Q: How can these antibodies help with neutralizing antibody detection in clinical samples?
A: For clinical applications, our antibodies can be used as key reagents in neutralizing antibody assays. These assays help determine if a patient has pre-existing antibodies that could inactivate the AAV vector, which is a critical step in patient stratification for gene therapy trials.
REFERENCE
- Rapti, Kleopatra, and Dirk Grimm. "Adeno-Associated Viruses (AAV) and Host Immunity - A Race Between the Hare and the Hedgehog." Frontiers in immunology vol. 12 753467. 29 Oct. 2021, Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3389/fimmu.2021.753467
