CALCRL Specific Neutra™ Antibody Products

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Accelerate Your Research and Development!

Are you currently facing challenges in precisely characterizing G protein-coupled receptor (GPCR) signaling, understanding neurovascular regulation, or developing targeted therapeutics for conditions like migraine or lymphedema? Our CALCRL Specific Antibody Products at Creative Biolabs help you achieve accurate and reliable detection and analysis of the Calcitonin Receptor-Like Receptor, enabling robust research into its complex biological roles and therapeutic potential through highly specific and validated antibody tools.

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Introduction of CALCRL

The Calcitonin Receptor-Like Receptor (CALCRL), also known as CRLR, is a fascinating human protein that plays a pivotal role in diverse physiological functions through its unique interaction with Receptor Activity-Modifying Proteins (RAMPs). Unlike typical G protein-coupled receptors (GPCRs), CALCRL requires association with one of three RAMPs (RAMP1, RAMP2, or RAMP3) to form functionally distinct receptors with specific ligand binding profiles.

Structure and Function: CALCRL is a single transmembrane domain receptor. When CALCRL associates with RAMP1, it forms the calcitonin gene-related peptide (CGRP) receptor. With RAMP2, it forms the adrenomedullin (AM) receptor (AM1), and with RAMP3, it forms a dual CGRP/AM receptor (AM2). These receptor complexes are primarily linked to the Gs protein, leading to the activation of adenylate cyclase and subsequent generation of intracellular cyclic adenosine monophosphate (cAMP), a key second messenger.
Signaling Pathways: The activation of these CALCRL-RAMP complexes initiates signaling cascades involved in a multitude of biological processes. The CGRP receptor, for instance, is widely distributed throughout the body, modulating functions in the respiratory, endocrine, gastrointestinal, immune, and cardiovascular systems. Adrenomedullin receptors are crucial for cardiovascular homeostasis, regulating blood pressure and vascular tone.
Related Diseases: Dysregulation of CALCRL signaling is implicated in several disease states. Notably, the CGRP receptor is a well-established target in migraine treatment, with CGRP receptor antagonists being approved for this condition. Furthermore, the CALCRL gene is implicated in hereditary lymphedema, highlighting its importance in lymphatic system development and function. Understanding these complex interactions is vital for both basic research and the development of targeted therapeutic strategies.

Fig 1. Schematic presentation of signaling pathways activated by CGRP receptor. (OA Literature) Fig. 1 Signaling pathways activated by CGRP receptor.¹

Antibodies Against CALCRL

Antibodies specifically targeting CALCRL are indispensable tools for advancing research into this critical receptor and its associated signaling pathways. Given CALCRL's dependence on RAMPs for functional expression and its involvement in various physiological and pathological processes, highly specific antibodies are crucial for accurate investigation.

Applications of CALCRL specific antibodies include:

Western Blotting (WB): To detect and quantify CALCRL protein expression levels in cell lysates and tissue homogenates, providing insights into its regulation under different physiological or pathological conditions.
Immunohistochemistry (IHC) and Immunofluorescence (IF): For precise localization of CALCRL within tissues and cells, revealing its cellular and subcellular distribution, and its co-localization with RAMPs or other interacting proteins. This is particularly valuable for understanding its role in specific cell types or anatomical structures.
Enzyme-Linked Immunosorbent Assay (ELISA): To develop sensitive assays for quantifying CALCRL protein levels in biological fluids or cell extracts, which can be useful for biomarker discovery or monitoring experimental manipulations.
Flow Cytometry (FACS): For analyzing CALCRL expression on the surface of various cell populations, enabling the characterization of cell types that express this receptor.
Immunoprecipitation (IP): To isolate CALCRL and its interacting partners, facilitating the study of protein-protein interactions within the receptor complex, particularly with RAMPs.

These applications are vital for elucidating the precise roles of CALCRL in health and disease, from its involvement in migraine pathogenesis to its broader impact on cardiovascular and lymphatic systems. Creative Biolabs’ CALCRL Specific Antibody Products are rigorously validated to ensure high affinity and specificity, providing researchers with reliable tools for their most demanding experiments.

Why Choose Us?

Creative Biolabs’ CALCRL Specific Antibody Products are meticulously developed to provide researchers with unparalleled precision in studying the Calcitonin Receptor-Like Receptor. These antibodies offer critical solutions for projects requiring accurate detection, quantification, and functional analysis of CALCRL and its associated receptor complexes (CGRP receptor, AM1, and AM2).

Our antibodies can assist your project by:

Enabling Precise Detection: Facilitate the accurate identification and localization of CALCRL protein in various biological samples, including tissues and cells, using techniques such as Western Blotting, Immunohistochemistry (IHC), and Immunofluorescence (IF).
Supporting Functional Studies: Aid in understanding the expression patterns and cellular distribution of CALCRL, which is crucial for investigating its role in diverse physiological processes, from cardiovascular regulation to bone metabolism and pain signaling.
Advancing Disease Research: Provide essential tools for exploring CALCRL's involvement in pathologies such as migraine (via CGRP receptor antagonism) and hereditary lymphedema, allowing for deeper insights into disease mechanisms and potential therapeutic targets.
Facilitating Drug Discovery: Serve as reliable reagents for screening and validating compounds that modulate CALCRL activity, supporting the development of novel therapeutics.

FAQs

Q: What are the primary applications for antibodies targeting the Calcitonin Receptor-Like Receptor?

A: Antibodies against this receptor are highly versatile and suitable for a broad range of applications, including Western Blotting for protein expression analysis, Immunohistochemistry and Immunofluorescence for cellular and tissue localization, and ELISA for quantitative detection. They are invaluable for studying receptor expression, signaling pathways, and involvement in various physiological and pathological conditions.

Q: How do these antibodies help in understanding the receptor's function in different physiological systems?

A: By allowing precise detection and localization of the receptor, these antibodies enable researchers to map its distribution in various tissues and cell types. This is crucial for elucidating its specific roles in different systems, such as its involvement in cardiovascular regulation, pain perception (via CGRP signaling), and lymphatic development, providing a clearer picture of its diverse biological functions.

Q: Can these antibodies differentiate between the various functional receptor complexes formed by the Calcitonin Receptor-Like Receptor with RAMPs?

A: While a single antibody may target the core receptor, its utility in differentiating specific functional complexes (e.g., CGRP receptor vs. AM1 receptor) often depends on the experimental design and the availability of specific RAMP antibodies. Researchers commonly use co-immunoprecipitation or co-localization studies with antibodies against individual RAMPs to fully characterize the specific receptor complex being studied.

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REFERENCE

Sánchez, Manuel Lisardo et al. “Peptidergic Systems and Cancer: Focus on Tachykinin and Calcitonin/Calcitonin Gene-Related Peptide Families.” Cancers vol. 15,6 1694. 9 Mar. 2023, DOI:10.3390/cancers15061694. Distributed under Open Access license CC BY 4.0, without modification.