Collagen Specific Neutra™ Antibody Products

Accelerate Your Research and Development!

Are you currently facing challenges in precisely characterizing extracellular matrix components, investigating tissue remodeling, or developing accurate diagnostics for fibrotic diseases and cancer? Our Collagen Specific Antibody Products from Creative Biolabs help you achieve unparalleled precision and reliability in detecting and quantifying various collagen types, enabling robust research into tissue biology, disease progression, and therapeutic development through highly specific antibodies and advanced immunoassay tools.

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Introduction of Collagen

Collagen, etymologically from Greek "kolla" denoting glue, represents mammals' predominant protein, accounting for roughly 25%–35% of complete somatic protein. Serving as the principal scaffolding protein in connective tissues' extracellular matrix (ECM), it confers toughness, pliability, and structural coherence to varied organs and tissues—encompassing skin, bone, tendon, ligament, cartilage, and vascular systems.

Structurally, collagen is characterized by its signature triple-helical conformation. Each molecule comprises three α polypeptide chains, typically abundant in glycine, proline, and hydroxyproline. These α-chains form a stable right-handed triple helix. The repeating Gly-X-Y sequence—where X frequently denotes proline and Y hydroxyproline—is essential for this helical assembly. No fewer than 28 collagen forms are recognized, each exhibiting distinctive structural properties, tissue-specific distribution, and functional purposes.

Fig. 1 Structure of collagen.Distributed under CC BY-SA 4.0, from Wiki, without modification

Collagen plays a crucial role in numerous biological processes beyond structural support. It is involved in cell adhesion, migration, differentiation, tissue development, and wound healing. Its interactions with various cell surface receptors, such as integrins and discoidin domain receptors (DDRs), mediate critical signaling pathways that influence cell behavior.

Dysregulation of collagen synthesis, degradation, or organization is implicated in a wide range of diseases. These include:

• Fibrotic Diseases: Excessive accumulation of collagen leads to tissue scarring and organ dysfunction in conditions like liver cirrhosis, pulmonary fibrosis, and kidney fibrosis.
• Cancer: The tumor microenvironment, rich in collagen, significantly influences tumor growth, invasion, and metastasis. Altered collagen architecture can promote cancer progression and affect therapeutic response.
• Autoimmune Diseases: Conditions like Systemic Sclerosis (scleroderma) are characterized by excessive collagen deposition.
• Genetic Disorders: Mutations in collagen genes can lead to diseases such as Ehlers-Danlos syndrome (affecting connective tissue elasticity) and osteogenesis imperfecta (brittle bone disease).
• Aging: The decline in collagen quality and quantity contributes to skin aging, joint issues, and reduced tissue elasticity.

Understanding the intricate biology of collagen is fundamental for developing therapeutic strategies for these debilitating conditions.

Antibodies Against Collagen

Antibodies specifically targeting collagen are indispensable tools in biological and medical research, offering highly precise methods for detecting, quantifying, and characterizing various collagen types in diverse biological contexts. These antibodies are developed to recognize specific epitopes on collagen molecules, allowing researchers to differentiate between collagen types, identify post-translational modifications, and assess collagen integrity.

Fig. 2 CBD-modified antibody targeting tumor collagen.¹

The applications of collagen-specific antibodies are extensive and critical across multiple disciplines:

• Immunohistochemistry (IHC) and Immunofluorescence (IF): These techniques utilize collagen antibodies to visualize the spatial distribution and organization of different collagen types within tissues. This is vital for studying tissue architecture, developmental biology, and pathological changes in diseases like fibrosis and cancer. For example, researchers can use anti-collagen I antibodies to identify areas of increased fibrosis in liver biopsies or anti-collagen IV antibodies to assess basement membrane integrity in kidney disease.
• Western Blotting: Collagen antibodies are used to detect and quantify specific collagen types in protein extracts, allowing for analysis of collagen expression levels and processing.
• Enzyme-Linked Immunosorbent Assay (ELISA): ELISA platforms employing collagen antibodies enable the sensitive and quantitative measurement of soluble collagen fragments or intact collagen molecules in biological fluids (e.g., serum, urine) or tissue homogenates. This is particularly useful for biomarker discovery and monitoring disease progression, such as assessing collagen turnover markers in fibrotic conditions.
• Flow Cytometry: For cell-based assays, collagen antibodies can be used to detect intracellular or surface-bound collagen, providing insights into cellular collagen synthesis and interaction with the ECM.
• Functional Studies: In some cases, antibodies can be used to block collagen-receptor interactions or modulate collagen-related signaling pathways, offering tools for functional investigations in cell culture or in vivo models.

The specificity of these antibodies is paramount, as different collagen types often coexist and have distinct roles. High-quality collagen antibodies ensure accurate differentiation and reliable results, making them essential for advancing our understanding of ECM biology and its implications in health and disease.

Why Choose Us?

Creative Biolabs’ Collagen Specific Antibody Products are engineered to provide precise and reliable tools for your most demanding research and diagnostic needs. We offer solutions that enable accurate detection and quantification of various collagen types across a multitude of biological samples and applications. Our antibodies are instrumental in studies involving tissue development, wound healing, fibrosis, cancer progression, and autoimmune diseases.

Specifically, our products can assist your project by:

• Precise Quantification of Collagen Levels: Our antibodies allow for accurate measurement of specific collagen types in complex biological matrices, crucial for understanding disease states and therapeutic efficacy.
• Characterization of Tissue Structure and Remodeling: Utilize our antibodies in immunohistochemistry and immunofluorescence to visualize collagen distribution and assess changes in tissue architecture during development, injury, or disease.
• Investigation of Fibrotic Processes: Gain deeper insights into the mechanisms of fibrosis in organs such as the liver, lung, and kidney by specifically targeting and quantifying collagen deposition.
• Support for Cancer Research: Explore the role of the collagen-rich tumor microenvironment in cancer progression, metastasis, and drug resistance.
• Development of Diagnostic Assays: Our high-specificity antibodies are ideal for creating robust diagnostic platforms for various collagen-related disorders.

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REFERENCE

1. Liu, Jiayang et al. “Targeting collagen in tumor extracellular matrix as a novel targeted strategy in cancer immunotherapy.” Frontiers in oncology vol. 13 1225483. 24 Aug. 2023, DOI:10.3389/fonc.2023.1225483. Distributed under Open Access license CC BY 4.0, without modification.