Dirofilaria immitis Specific Neutra™ Antibody Products
Product listAccelerate Your Research and Development!
Are you currently facing long development cycles for diagnostic assays, low sensitivity in detecting early-stage infections, or challenges with cross-reactivity in heartworm detection platforms? Our D. immitis Specific Neutra™ Antibody Products help you develop highly accurate, sensitive, and rapid diagnostic tools through advanced nematode antigen specificity and rigorous validation.
Contact our team to get an inquiry now!
Introduction of D. immitis
Dirofilaria immitis, or heartworm, is a serious, often fatal, mosquito-borne filarial nematode globally impacting definitive hosts like domestic dogs. Its life cycle involves mosquitoes (intermediate hosts, L1 to L3) and mammals. Infective L3 larvae migrate, developing into adult worms (up to 30 cm) that reside in the pulmonary arteries, where they mate and release microfilariae (L1). The resulting pathology—caused by the physical presence of adult worms and inflammation—includes pulmonary artery thickening, pulmonary hypertension, and right-sided heart failure (cor pulmonale). Canine diagnosis primarily relies on detecting the circulating, heat-stable antigen secreted by mature female worms. Capturing this key antigen, which includes specific immunogenic polypeptides, requires high-performance diagnostic antibodies.
Distributed under CC BY-SA 3.0, from Wiki, without modification Fig.1 Dirofilaria immitis lifecycle.
Antibodies Against D. immitis
High-specificity anti-D. immitis antibodies are essential for advanced diagnostics, allowing for precision antigen binding and the detection of occult infections.
Strategic Antibody Utilization
- Antigen Capture and Detection: We engineer high-performance monoclonal antibodies (mAbs) for use as specific capture/detection pairs in quantitative and qualitative immunoassays, ensuring reproducible detection of a single epitope to maximize signal-to-noise ratio.
- Investigating Immune Complexes: Because immune complex formation can mask target antigens and cause false-negatives, our antibodies are optimized for assays that use pre-treatment steps (heat/acid) to release the antigen for subsequent binding.
- Research Applications: Polyclonal antibodies (pAbs) are vital for broader epitope recognition, suitable for research techniques like Immunohistochemistry, Immunofluorescence (for localizing parasite components), and Western Blotting (to characterize new antigenic proteins).
Why Choose Us?
Creative Biolabs' D. immitis Specific Neutra™ Antibody Products result from two decades of dedication, overcoming common heartworm diagnostic pitfalls.
Creative Biolabs' Distinct Advantages:
- Neutra™ Specificity: Rigorous cross-screening ensures highest specificity against non-target nematodes, preventing false positives.
- Superior Affinity: Optimized for robust detection of trace circulating antigens, critical for low worm burden and prepatent identification.
- Consistency & Formats: Stringent QC guarantees lot-to-lot consistency. Reagents are available in multiple, ready-to-use conjugated formats (Biotin, HRP, fluorescent) for integration into ELISA, LF, WB, and IF platforms.
- Scientific Support: Our experienced team offers personalized support to troubleshoot assay development.
FAQs
Q: Can these antibodies reliably detect infection in animals with low parasite burdens?
A: Yes. One focus was to enhance the limit of detection (LOD). Our high-affinity capture and detection pairs are specifically engineered to provide superior binding kinetics, which significantly improves the detection rate in samples containing low concentrations of circulating antigen, offering greater assurance in screening potentially asymptomatic animals.
Q: What is the optimal assay platform to maximize the performance of these reagents?
A: While these antibodies are versatile, performance is maximized in a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) format using a high-binding plate. This platform allows capture and detection antibodies to work in concert, amplifying the signal for the target antigen. They are also highly effective in point-of-care Lateral Flow assays (LFIA) requiring rapid results and robust signal stability.
Q: How do these antibodies perform when detecting prepatent infections (before adult worms mature)?
A: Antigen detection tests, by nature, rely on the production of uterine antigen by mature female worms. While no antigen test can detect the parasite immediately post-infection, these antibodies are validated to reliably detect antigen as early as five to six months post-infection, aligning with the onset of patency. For earlier detection, alternative immunological targets should be considered.
Q: Is there a risk of cross-reactivity with other common filarial or non-filarial parasites?
A: Specificity is non-negotiable. Our rigorous screening protocol includes extensive testing against antigens from related nematodes and common commensals. This meticulous approach reduces the likelihood of false positives and ensures data integrity, significantly simplifying your validation process.
Q: Beyond standard detection, are these reagents suitable for quantitative analysis?
A: Absolutely. The consistency and high purity of these antibodies make them ideal for quantitative ELISA and other platforms. When utilized with appropriate standards and controls, they allow for reliable semi-quantitative or quantitative measurement of circulating antigen levels, which can be invaluable for monitoring the efficacy of therapeutic interventions and tracking disease progression in research models.
Access the Creative Biolabs Edge – Request Your Quote Now
