FGFR4 Specific Neutra™ Antibody Products
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Are you currently facing challenges in accurately characterizing Fibroblast Growth Factor Receptor 4 (FGFR4) in complex biological systems, or struggling with the development of highly specific and reliable tools for cancer research and therapeutic development? Creative Biolabs’ FGFR4 Specific Antibody Products help you achieve precise and reliable FGFR4 detection and modulation, enabling robust research into related signaling pathways and therapeutic potential through advanced immunoassay tools and high-specificity antibodies.
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Introduction of FGFR4
Fibroblast Growth Factor Receptor 4 (FGFR4) is a member of the fibroblast growth factor receptor (FGFR) family, a group of receptor tyrosine kinases that play crucial roles in cell proliferation, differentiation, migration, and survival. FGFR4 constitutes a membrane-spanning protein possessing an external ligand-binding region, one transmembrane helix, and a cytoplasmic tyrosine kinase segment. It is activated by binding to specific fibroblast growth factors (FGFs), particularly FGF19, FGF2, and FGF8.
Upon ligand binding, FGFR4 undergoes dimerization, leading to autophosphorylation of tyrosine residues within its intracellular domain. This phosphorylation launches a sequence of downstream signaling activities, centrally engaging the RAS/MAPK, PI3K/AKT, and PLCγ routes. These pathways govern multiple biological functions such as proliferation, viability, and metabolism.
Dysregulation of FGFR4 signaling has been implicated in various human diseases, most notably in several types of cancer. Overexpression or activating mutations of FGFR4 are frequently observed in hepatocellular carcinoma (HCC), rhabdomyosarcoma, breast cancer, and prostate cancer. In these malignancies, aberrant FGFR4 activity can promote tumor cell proliferation, survival, angiogenesis, and metastasis, making it a promising target for therapeutic intervention. Its role in bile acid metabolism, particularly through the FGF19-FGFR4 axis, also highlights its importance in metabolic disorders.
Fig. 1 The FGF/FGFR4 signal axis.1
Antibodies Against FGFR4
Antibodies targeting FGFR4 are invaluable tools in both basic research and clinical applications. These antibodies can be developed to recognize various epitopes on the FGFR4 protein, including its extracellular domain for blocking ligand binding, its intracellular domain for detecting total protein levels, or specific phosphorylation sites to monitor activation status.
The applications of FGFR4 antibodies are extensive:
- Western Blotting (WB): For detecting and quantifying FGFR4 protein expression levels in cell lysates and tissue samples.
- Immunohistochemistry (IHC) and Immunofluorescence (IF): To visualize the cellular localization and expression patterns of FGFR4 in tissue sections and cultured cells, crucial for understanding its role in disease pathology.
- Enzyme-Linked Immunosorbent Assay (ELISA): For quantitative measurement of FGFR4 levels in biological fluids or cell lysates, useful for biomarker discovery and drug screening.
- Flow Cytometry (FACS): To analyze FGFR4 expression on the surface of live cells, enabling cell population characterization and sorting.
- Functional Studies: Blocking antibodies can be used to inhibit FGFR4 signaling, providing insights into its biological functions and therapeutic potential in cancer models. Agonistic antibodies might be used to activate the receptor for specific research purposes.
- Therapeutic Development: Antibodies against FGFR4 are being investigated as potential therapeutic agents, particularly in cancers where FGFR4 is overactive.
These antibodies provide critical insights into FGFR4's biological roles, its involvement in disease progression, and its potential as a therapeutic target. Creative Biolabs’ FGFR4 Specific Antibody Products provide robust and reliable solutions for your research, diagnostic, and therapeutic development needs. With our commitment to quality, specificity, and expert support, we empower scientists to accelerate their understanding of FGFR4's complex biology and its role in disease.
Why Choose Us?
At Creative Biolabs, we are committed to providing researchers with the highest quality FGFR4 Specific Antibody Products, backed by extensive expertise and rigorous validation. Our commitment to excellence sets us apart:
- Unparalleled Specificity and Sensitivity: Our FGFR4 antibodies are rigorously developed and validated to ensure minimal cross-reactivity with other FGFR family members or unrelated proteins, providing highly accurate and reliable results. We optimize for exceptional sensitivity, enabling the detection of even low concentrations of FGFR4.
- Comprehensive Validation: Each antibody undergoes stringent validation across multiple applications (e.g., WB, IHC, IF, ELISA) to ensure consistent performance and reproducibility.
- Expert Support: Our team of experienced scientists is dedicated to providing comprehensive technical support, assisting you with experimental design, troubleshooting, and data interpretation.
- Customization Capabilities: Beyond our catalog products, Creative Biolabs specializes in custom antibody development. For projects needing antibodies with defined attributes—like specific host species, isotype, or affinity for particular FGFR4 variants—our expert team develops tailored antibody solutions matching your exact requirements.
FAQs
Q: How can I ensure the optimal performance of these antibodies in my specific assay?
A: To achieve the best results, we recommend optimizing key parameters such as antibody concentration, incubation times, and blocking conditions. Detailed protocols are provided with each product, and our scientific support team is available to assist with any specific experimental design questions.
Q: Are these antibodies suitable for detecting both native and recombinant forms of the target?
A: Our antibodies are generally designed to recognize both native and recombinant forms of the target protein. However, specific performance may vary depending on the conformation and post-translational modifications of your particular sample.
Q: What are the primary advantages of using antibody-based detection methods for this target compared to other techniques?
A: Antibody-based immunoassays offer several significant advantages, including high sensitivity for detecting low-abundance proteins, excellent specificity to minimize false positives, and versatility across various applications (e.g., Western Blotting, IHC, ELISA). They are also amenable to high-throughput screening, making them efficient for large sample sets.
Q: Can these antibodies be used in functional studies, such as blocking or activation assays?
A: Some of our antibodies are characterized for their ability to block or activate receptor function. Please refer to the individual product datasheets for information on functional validation and recommended applications. If you have specific functional study needs, our team can help you identify the most suitable antibody.
Q: What considerations should I keep in mind regarding sample preparation when using these antibodies?
A: Adherence to standardized specimen handling protocols determines analytical precision. Depending on the application, this may involve specific lysis buffers, protease and phosphatase inhibitors, or appropriate tissue fixation methods. Always follow established protocols for your sample type and consult the product datasheet for any specific recommendations.
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REFERENCE
- Lang, Liwei, and Yong Teng. “Fibroblast Growth Factor Receptor 4 Targeting in Cancer: New Insights into Mechanisms and Therapeutic Strategies.” Cells vol. 8,1 31. 9 Jan. 2019, DOI:10.3390/cells8010031. Distributed under Open Access license CC BY 4.0, without modification.
