HAVCR1 Specific Neutra™ Antibody Products
Product listAccelerate Your Research and Development!
Are you currently facing challenges in accurately detecting and quantifying HAVCR1 in complex biological matrices, or struggling to develop highly sensitive immunoassays for disease biomarker discovery and therapeutic development? Creative Biolabs’ HAVCR1 Specific Antibody Products help you achieve precise and reliable HAVCR1 detection, enabling robust research into its diverse biological roles and clinical implications through advanced immunoassay tools and high-specificity antibodies.
Contact our team to get an inquiry now!
Introduction of HAVCR1
Hepatitis A Virus Cellular Receptor 1 (HAVCR1), also widely known as T-cell immunoglobulin and mucin domain 1 (TIM-1) or Kidney Injury Molecule-1 (KIM-1), is a multifaceted type I transmembrane glycoprotein. It is encoded by the HAVCR1 gene in humans and plays crucial roles in both immune regulation and tissue injury.
Structure
Architecturally, HAVCR1 is categorized within the T-cell immunoglobulin and mucin domain (TIM) protein family. It features an N-terminal immunoglobulin (Ig)-like domain, a mucin domain, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This unique architecture allows it to participate in diverse biological interactions.
Function
Functionally, it serves as a receptor for human hepatitis A virus (HHAV) and TIMD4. Beyond HAV, it is also recognized as a receptor or cofactor for the entry of multiple other viruses, including Ebola virus, Marburg virus, Dengue virus, and Zika virus, and has been identified as a possible entry factor for SARS-CoV-2 and other coronaviruses. Immunologically, TIM-1 (HAVCR1) shows predominant expression on Th2 cells, operating as an activation cofactor for T-cells that modulates allergy, asthma, and transplant acceptance. Its role as a receptor for phosphatidylserine also enables it to bind to apoptotic cells, facilitating their clearance.
Signaling Pathways
HAVCR1 is involved in various signaling pathways, including those related to viral entry and infection, immune response modulation, and kidney injury. For instance, it mediates hepatitis A virus infection and enhances HCV infection. Emerging research also links its activity to the PI3K/AKT/p53 pathway in cervical cancer.
Fig. 1 Life cycle of the hepatitis A virus.1
Physiological and Pathological Processes
The involvement of HAVCR1 in various physiological and pathological processes makes it a significant target in disease research. It is associated with a broad spectrum of diseases, including hepatitis A, asthma, allergic diseases, and other viral infections (Ebola, Marburg, Dengue, Zika, SARS-CoV-2). Notably, HAVCR1 (KIM-1) is the most highly upregulated type 1 transmembrane protein in injured kidneys due to various insults, making it a critical biomarker for kidney injury and conditions like lupus nephritis and diabetic nephropathy. Furthermore, it has been implicated in obstructive sleep apnea-hypopnea syndrome, dilated cardiomyopathy-induced heart failure, stroke, type 1 diabetes, preeclampsia, and several cancers including prostate cancer, gastric cancer, and clear cell renal carcinoma.
Antibodies Against HAVCR1
HAVCR1-specific antibodies are indispensable tools for research and diagnostics, designed for high-affinity, selective binding to enable precise detection and quantification in diverse biological contexts. Their applications are diverse and impactful across research and clinical fields:
- Immunoassay Development: For immunoassay development (e.g., ELISA), they enable highly sensitive and specific quantitative detection of soluble HAVCR1 in biological fluids or cell lysates.
- Western Blotting (WB): In Western Blotting, they detect and characterize HAVCR1 protein expression in cell and tissue extracts, revealing regulatory insights.
- Immunohistochemistry (IHC) and Immunofluorescence (IF): IHC and IF applications allow visualization of HAVCR1 expression and localization in tissues and cells, valuable for studying its distribution in kidney injury, immune populations, and viral infection sites.
- Flow Cytometry (FACS): Flow Cytometry utilizes these antibodies to identify and quantify HAVCR1-expressing cell populations (e.g., activated T cells, kidney epithelial cells), aiding studies on immune subsets and cellular responses.
- Biomarker Research: As a prominent kidney injury biomarker, HAVCR1 antibodies are essential for its detection in urine and plasma, supporting early diagnosis, prognosis, and disease monitoring.
- Viral Research: In viral research, they are critical for investigating HAVCR1's function as a viral receptor, elucidating viral entry mechanisms and host-pathogen interactions for viruses like Hepatitis A, Ebola, and Dengue.
Why Choose Us?
Creative Biolabs’ HAVCR1 Specific Antibody Products are meticulously developed to provide unparalleled precision and reliability for your research and diagnostic needs. We offer specific solutions for detecting HAVCR1, enabling researchers to accurately quantify protein presence, study its expression profiles, and develop robust diagnostic tools. Our solutions facilitate the development of sensitive immunoassays, support biomarker analysis in various diseases, and aid in understanding the biological impact of HAVCR1 in immune responses, viral infections, and kidney injury.
Specific deliverables and solutions include:
- Accurate Detection and Quantification: Our antibodies enable the precise measurement of HAVCR1 levels in diverse sample types, including cell lysates, tissue sections, and biological fluids.
- Biomarker Discovery and Validation: Facilitate the identification and validation of HAVCR1 as a potential biomarker for kidney injury, autoimmune diseases, and viral infections.
- Mechanistic Studies: Support investigations into HAVCR1's role in immune regulation, T-cell activation, viral entry mechanisms, and disease pathogenesis.
- Diagnostic Assay Development: Provide high-quality reagents essential for developing sensitive and specific diagnostic kits for various clinical applications.
- Immunohistochemistry and Immunofluorescence: Enable visualization of HAVCR1 expression patterns in tissues and cells, offering insights into its cellular localization and distribution.
FAQs
Q: What are the primary applications for antibodies against this target?
A: Antibodies against this target are highly versatile and widely used in various applications, including Enzyme-Linked Immunosorbent Assay (ELISA), Western Blotting, Immunohistochemistry (IHC), Immunofluorescence (IF), and flow cytometry. They are particularly valuable for studying protein expression, localization, and quantification in research contexts, and for developing diagnostic assays.
Q: How do these antibodies contribute to understanding disease mechanisms?
A: By specifically binding to the target protein, these antibodies enable researchers to precisely detect and quantify its presence in biological samples. This allows for the investigation of its expression levels in healthy versus diseased states, its cellular localization, and its involvement in specific signaling pathways. Such insights are crucial for elucidating the underlying mechanisms of various diseases, including inflammatory conditions, viral infections, and tissue injuries.
Q: How do these antibodies compare to other detection methods for this target?
A: Antibody-based detection methods offer several advantages, including high specificity and sensitivity, which are crucial for accurate and reliable results, especially when dealing with complex biological samples or low protein concentrations. They allow for direct visualization of the target in situ (IHC/IF) and provide quantitative data (ELISA, WB), offering a comprehensive approach compared to some non-antibody-based techniques.
Access the Creative Biolabs Edge – Request Your Quote Now
REFERENCE
- Kanda, Tatsuo et al. “Cell Culture Systems and Drug Targets for Hepatitis A Virus Infection.” Viruses vol. 12,5 533. 12 May. 2020, DOI:10.3390/v12050533. Distributed under Open Access license CC BY 4.0, without modification.
