iPS/ES Specific Neutra™ Antibody Products
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Creative Biolabs’ iPS/ES Specific Antibody Products are designed to provide researchers with unparalleled precision in identifying, isolating, and characterizing induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs). Our solutions enable robust quality control, facilitate differentiation studies, and support the development of safe and effective cell-based therapies. We deliver highly specific and validated antibody reagents, comprehensive data to support your findings, and expert technical guidance to ensure your project's success.
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Introduction of iPS/ES
Induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs) represent a cornerstone of modern regenerative medicine and disease modeling. Both cell types possess the remarkable ability of pluripotency, meaning they can self-renew indefinitely and differentiate into virtually any cell type of the human body. ESCs are derived from the inner cell mass of a blastocyst, an early-stage embryo, a process that historically raised ethical considerations due to the destruction of the embryo. In contrast, iPSCs are generated by reprogramming adult somatic cells, such as skin fibroblasts, back into a pluripotent state, thereby circumventing the ethical concerns associated with ESCs.
Fig. 1 Induced pluripotent stem cell (iPSC)-derived cellular models.1
While iPSCs and ESCs exhibit core stemness properties, current investigations persistently probe nuanced differences in their transcriptional signatures. Ensuring quality and controlled lineage commitment necessitates sophisticated analytical approaches. These encompass stringent evaluations like teratoma assays and chimera/tetraploid complementation, supplemented by genome-wide reference scorecards. Additionally, epigenetic alterations—specifically DNA/chromatin methylation signatures—play indispensable roles in directing cellular specialization and reprogramming. Accurate identification and profiling of these cells and their derived lineages remain essential for safe, effective diagnostic and therapeutic translation.
Antibodies Against iPS/ES
Antibodies targeting specific cell surface proteins and intracellular markers of iPSCs and ESCs are indispensable tools for stem cell research. These antibodies enable researchers to:
- Identify and Characterize Pluripotent Stem Cells: Antibodies against key pluripotency markers such as POU5F1 (OCT4), SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 are routinely used to confirm the pluripotent state of iPSCs and ESCs. The development of new monoclonal antibodies, as highlighted in recent research, specifically targeting defined cell surface proteins on human pluripotent stem cells, offers enhanced precision for these characterization efforts. These novel antibodies, often showing high correlation with OCT4 expression, are valuable for detecting rare pluripotent cells within mixed populations.
- Isolate and Enrich Specific Cell Populations: Flow cytometry and magnetic-activated cell sorting (MACS) using iPS/ES specific antibodies allow for the efficient isolation and enrichment of pluripotent stem cells from heterogeneous cultures, or the removal of undifferentiated cells from differentiated populations, which is critical for safety in therapeutic applications.
- Monitor Differentiation: Antibodies against lineage-specific markers can be used to track the differentiation process of iPSCs and ESCs into various cell types, ensuring the purity and identity of the desired differentiated cells.
- Quality Control: Regular antibody-based assays are essential for the quality control of stem cell lines used in research and clinical applications, ensuring consistency and preventing the presence of tumorigenic undifferentiated cells.
- Investigate Pluripotency Mechanisms: Antibodies reactive to both primed and naive states of hPSCs provide critical tools for understanding the intricate cellular mechanisms underlying human pluripotency and the processes of cellular reprogramming. Furthermore, neutralizing antibodies against iPS/ES cells can be invaluable for studying cell-cell interactions and signaling pathways crucial for maintaining pluripotency or inducing differentiation.
Why Choose Us?
Creative Biolabs stands at the forefront of stem cell research, offering iPS/ES Specific Antibody Products that deliver unmatched reliability and performance. Our commitment to scientific excellence and customer success sets us apart.
- Unrivaled Specificity and Sensitivity: Our antibodies are rigorously validated against a diverse panel of iPS/ES cell lines and differentiated cells, ensuring high specificity and sensitivity for accurate detection of pluripotent markers. This minimizes off-target binding and false positives, crucial for reliable research outcomes.
- Comprehensive Validation: Each antibody undergoes extensive validation across multiple applications (e.g., immunofluorescence, flow cytometry, western blot), providing confidence in their performance for your specific experimental needs. Published data have consistently demonstrated the superior performance of our antibodies in various research settings.
- Expert Scientific Support: Our team comprises seasoned biologists and immunologists ready to provide in-depth technical assistance, experimental design advice, and troubleshooting support, ensuring you maximize the utility of our products.
- Ethical Sourcing and Production: We adhere to the highest ethical standards in our production processes, particularly relevant for products related to stem cell research, ensuring responsible scientific advancement.
- Accelerated Research: By providing highly characterized and reliable reagents, we help researchers save valuable time and resources that would otherwise be spent on extensive in-house validation, allowing for faster progress in their studies.
FAQs
Here are some common questions from researchers interested in iPS/ES specific antibody products:
Q: How do Creative Biolabs’ iPS/ES Specific Antibody Products ensure high specificity for pluripotent cells?
A: Our antibodies undergo rigorous validation against a wide range of iPS and ES cell lines, as well as various differentiated cell types. This extensive testing, often involving multiple applications like immunofluorescence and flow cytometry, confirms their precise binding to pluripotent markers while minimizing cross-reactivity with non-pluripotent cells. This meticulous approach ensures you get highly specific and reliable results for your research.
Q: Can these antibodies be used for both human and animal iPS/ES cells?
A: While many of our iPS/ES Specific Antibody Products are optimized for human pluripotent stem cells, we also offer a selection validated for other species, including equine models, as discussed in relevant scientific literature. We recommend checking the specific product data sheet or contacting our technical support team to confirm compatibility with your target species and application.
Q: What applications are Creative Biolabs’ iPS/ES Specific Antibody Products validated for?
A: Our antibodies are typically validated for common stem cell research applications, including but not limited to immunofluorescence (IF), flow cytometry (FC), and Western blotting (WB). Some antibodies may also be suitable for immunohistochemistry (IHC), immunoprecipitation (IP), or other specialized assays. Detailed application data and recommended protocols are provided with each product.
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REFERENCE
- Cerneckis, Jonas et al. “Induced pluripotent stem cells (iPSCs): molecular mechanisms of induction and applications.” Signal transduction and targeted therapy vol. 9,1 112. 26 Apr. 2024, DOI:10.1038/s41392-024-01809-0. Distributed under Open Access license CC BY 4.0, without modification.
