Listeria monocytogenes p60 Specific Neutra™ Antibody Products
Product listAccelerate Your Research and Development!
Are you currently facing challenges in the specific and rapid detection of Listeria monocytogenes in complex food or environmental samples, or struggling to produce high-affinity antibodies for next-generation immunoassays? Our L. monocytogenes p60 Specific Neutra™ Antibody Products help you achieve reliable, high-sensitivity identification of this critical foodborne pathogen through advanced monoclonal antibody engineering focused on the highly-secreted p60 virulence factor.
Contact our team to get an inquiry now!
Introduction of L. monocytogenes p60
Listeria monocytogenes (L. monocytogenes) is a ubiquitous Gram-positive bacterium that causes listeriosis, a serious foodborne illness with high mortality, especially in vulnerable populations. Its ability to survive in diverse environments and spread cell-to-cell makes it a major public health concern.
The p60 protein, encoded by the iap gene, is a major extracellular autolysin (murein hydrolase) essential for bacterial cell division and daughter cell separation. It is a 60 kDa protein containing LysM (for cell wall adhesion) and catalytic domains. Critically, p60 is secreted in large, stable quantities into the culture supernatant, making it an excellent, accessible target for immunological detection assays, unlike strictly intracellular proteins.
Beyond cell division, p60 is a significant virulence factor, contributing to the pathogen's adherence, host cell invasion, and sustained colonization. Its fragments can also modulate the innate immune response, such as stimulating Natural Killer (NK) cells.
Fig.1 Stages of the life cycle of L. monocytogenes.1
Antibodies Against L. monocytogenes p60
Monoclonal antibodies (MAbs) targeting L. monocytogenes p60 have become indispensable tools in both public health and research for their ability to provide rapid and accurate detection solutions.
Diagnostic and Research Applications of Anti-p60 Antibodies:
The utility of anti-p60 antibodies stems from p60's immunogenic nature and its abundant secretion.
| Application Area | Method | Contribution of Anti-p60 Antibodies |
| Food Safety & Diagnostics | Sandwich ELISA, Lateral Flow Assays | Used as high-affinity capture and/or detection reagents for rapid, high-throughput screening of food and environmental samples for L. monocytogenes contamination. |
| Pathogenesis Research | Western Blotting (WB) | Essential for confirming p60 expression and secretion in various strains and growth conditions, supporting virulence studies. |
| Cell Biology & Localization | Immunofluorescence (IF), IHC | Used to visualize the localization of p60 on the bacterial cell surface or in host-pathogen interaction models. |
| Immunological Studies | Neutralization/Inhibition Assays | Investigating the role of p60 in host cell invasion or cell-to-cell spread by neutralizing the protein's function. |
Our L. monocytogenes p60 Specific Neutra™ Antibodies are rigorously validated to ensure performance across these diverse applications, offering both species-specific antibodies (targeting epitopes unique to L. monocytogenes) and group-reactive antibodies (targeting conserved epitopes for pan-Listeria detection).
Why Choose Us?
Creative Biolabs offers years of expertise in microbial biology and immunoassay development, ensuring reliable diagnostic tools that accelerate your research timeline and improve reliability.
- Superior Specificity & Sensitivity: Advanced engineering minimizes cross-reactivity with non-pathogenic Listeria (reducing false positives) while high affinity detects picogram-level concentrations of p60, vital for early detection.
- Validated for Complex Matrices: Our antibodies are pre-validated to perform robustly in challenging matrices, including food enrichments and biological fluids.
- Guaranteed Consistency & Expert Support: Rigorous quality control provides reproducible performance (supported by Published Data), and expert consultation ensures seamless integration into your existing workflows.
FAQs
Q: How do these p60 antibodies facilitate a faster workflow?
A: Traditional confirmation takes days. Because p60 is highly secreted, our antibodies allow immunoassays (like ELISA) to detect the protein directly from early enrichment cultures, providing rapid presumptive identification.
Q: Can they differentiate virulent L. monocytogenes from non-pathogenic species?
A: Yes. Our antibodies are screened to target unique, species-specific epitopes on the L. monocytogenes p60 variant, minimizing cross-reactivity with species like L. innocua and ensuring high diagnostic specificity.
Q: What sample preparation is necessary?
A: An enrichment step in liquid culture is required for p60 secretion. Boiling the culture supernatant is often recommended to maximize protein release and ensure epitope accessibility.
Q: Are these antibodies compatible with on-site, rapid detection formats?
A: Yes. P60's stability and high concentration make it an excellent target for incorporation into rapid diagnostic technologies such as Lateral Flow Devices (LFDs) and immunosensors.
Q: How is p60 detected in situ within infected host cells?
A: Immunofluorescence (IF) or Immunohistochemistry (IHC) is recommended. The antibody binds to surface-associated p60, enabling visualization of the bacteria and tracking p60's role in host cell invasion.
Access the Creative Biolabs Edge – Request Your Quote Now
REFERENCE
- Wiktorczyk-Kapischke, Natalia et al. "Genomic and pathogenicity islands of Listeria monocytogenes-overview of selected aspects." Frontiers in molecular biosciences vol. 10 1161486. 14 Jun. 2023, Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3389/fmolb.2023.1161486
