Legionella pneumophila Specific Neutra™ Antibody Products
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Are you currently facing challenges in rapidly and accurately diagnosing Legionnaires' disease, or struggling to develop highly specific research tools to track L. pneumophila serogroups and virulence factors? Our L. pneumophilia Specific Neutra™ Antibody Products help you accelerate reliable pathogen detection and fundamental research through high-affinity monoclonal and polyclonal antibody technology targeting key surface antigens and secreted proteins.
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Introduction of L. pneumophilia
Legionella pneumophila (L. pneumophila) is a ubiquitous Gram-negative bacterium found in freshwater environments, where it survives inside free-living protozoa. When aerosolized and inhaled by humans, it causes severe pneumonia known as Legionnaires' disease, or the milder Pontiac fever, necessitating robust detection methods.
Its pathogenicity depends on its ability to infect and replicate within host alveolar macrophages. Upon being phagocytosed, L. pneumophila prevents phagosome-lysosome fusion and creates the Legionella-containing vacuole (LCV). This process is mediated by the Dot/Icm Type IV secretion system, which injects hundreds of effector proteins to hijack host cell processes (including trafficking and signaling) for bacterial growth. Diagnostics primarily rely on studying the bacterial surface structures, particularly the highly immunogenic Lipopolysaccharide (LPS) from serogroup 1.
Fig.1 The host cell–Legionella spp. interaction and the pathogenesis of LD.1
Antibodies Against L. pneumophilia
Antibodies targeting L. pneumophila are essential tools for clinical diagnostics and basic research, serving as precise probes for the organism's presence and activity. Key targets include:
- Lipopolysaccharide (LPS): The most common target, particularly serogroup 1 LPS, used for direct antigen detection (e.g., in urine) and serological assays to confirm infection and differentiate serogroups.
- Outer Membrane Proteins (OMPs): Used to study bacterial surface structure, viability, and host-environment interactions.
- Dot/Icm Effectors: Intracellular virulence factors studied via immunofluorescence and Western blotting to track effector protein localization and abundance within infected host cells.
Our Neutra™ Antibodies are validated for diverse applications: Direct Fluorescent Antibody (DFA) Tests (rapid on-site identification in clinical and environmental samples), Enzyme-Linked Immunosorbent Assay (ELISA) (high-throughput screening for patient serology and environmental water antigen detection), Western Blotting (WB)/Immunoprecipitation (IP) (characterizing bacterial protein expression and Dot/Icm effectors), and Flow Cytometry (assessing viability and tracking infection dynamics).
Why Choose Us?
As a specialist in legionellosis reagents, Creative Biolabs guarantees superior specificity, particularly for Serogroup 1 LPS, proven via stringent validation in complex environmental and clinical samples. The Neutra™ formulation ensures extended stability and batch consistency. We also provide expert technical support and custom conjugation (fluorophores, biotin, enzymes) for seamless integration into your diagnostic and research workflows. (Refer to Published Data showcasing high signal-to-noise ratios in DFA and ELISA.)
FAQs
Q: How can I ensure minimal cross-reactivity with non-pneumophila Legionella species?
A: High specificity is critical for accurate environmental monitoring. Select antibodies rigorously tested against a panel of non-target strains and other pathogens, with public data demonstrating exclusive binding to the target antigen, such as L. pneumophila Serogroup 1.
Q: Monoclonal vs. Polyclonal for assays like ELISA?
A: Monoclonals offer unparalleled specificity (single epitope binding) for reproducible quantitative assays. Polyclonals recognize multiple epitopes, yielding a stronger signal and higher sensitivity for initial screening or WB, but require more stringent cross-reactivity control.
Q: Are reagents suitable for complex sample types (e.g., cooling tower water, clinical secretions)?
A: Yes. Robust reagents are validated against purified antigen and tested in spiked/native complex matrices. Check for validation data showing performance against environmental inhibitors or in sputum/bronchial washings.
Q: Can these antibodies be used for functional studies (phagocytosis/trafficking)?
A: Absolutely. Antibodies targeting surface markers monitor bacterial uptake, while those against virulence factors are essential for immunofluorescence microscopy to track protein localization within the host cell.
Q: Can I obtain a customized conjugation of the antibody?
A: Yes, specialized custom conjugation services are offered. This allows high specificity to be maintained while adapting the antibody to unique fluorescent dyes or high-throughput platforms.
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REFERENCE
- Khweek, Arwa Abu, and Amal Amer. "Replication of Legionella Pneumophila in Human Cells: Why are We Susceptible?." Frontiers in microbiology vol. 1 133. 28 Dec. 2010, Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3389/fmicb.2010.00133
