Mycoplasma pneumoniae P1 Specific Neutra™ Antibody Products
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Are you currently facing challenges in achieving specific and reliable Mycoplasma pneumoniae detection, or struggling to develop high-performance diagnostic assays and effective infection-blocking therapeutics? Our M. pneumoniae P1 Specific Neutra™ Antibody Products help you accelerate diagnostic kit development and advance research into respiratory pathogenesis through antibodies engineered for high specificity and exceptional performance across diverse immunoassay platforms. We leverage advanced protein engineering techniques to ensure unparalleled quality.
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Introduction of M. pneumoniae P1
Mycoplasma pneumoniae is a common human respiratory pathogen responsible for millions of cases of community-acquired pneumonia, often referred to as "walking pneumonia," particularly in school-age children and young adults. Unlike other bacteria, M. pneumoniae lacks a cell wall, making it inherently resistant to many common antibiotics, such as β-lactams. Its pathogenesis is critically dependent on its ability to adhere tightly to the respiratory epithelium.
The P1 Adhesin is the primary, large-scale surface protein that mediates this essential attachment. The P1 protein, a significant virulence factor, is clustered at the leading edge of the specialized attachment organelle. Its function is to recognize and bind to sialylated oligosaccharide receptors present on the surface of host ciliated epithelial cells. This binding is not just for colonization; it also leads to the loss of ciliary motion (ciliostasis) and subsequent destruction of the epithelial cells, initiating the inflammatory response and clinical symptoms of pneumonia. The P1 Adhesin's critical role in infectivity, coupled with its highly immunogenic nature, makes it a pivotal target for both diagnostic assays and the development of effective, non-antibiotic intervention strategies, as widely confirmed across cited literature.
Fig.1 Pathogenic mechanisms of M. pneumoniae.1
Antibodies Against M. pneumoniae P1
P1 Adhesin is an ideal, essential target for tools spanning fundamental research to clinical diagnostics. Our Neutra™ Antibody Products target distinct P1 epitopes for comprehensive utility. Key applications include:
- Diagnostics: Routinely used in serological tests (to detect patient antibodies) or to directly capture the P1 antigen in clinical samples, requiring the high affinity and specificity of the Neutra™ line.
- Functional Assays: Employed in Inhibition of Attachment Assays, where neutralizing antibodies block P1 adhesion to host cells in vitro. This is indispensable for screening therapeutic and vaccine candidates that mimic or enhance the natural immune response.
- Antigen Characterization: Vital for mapping P1 structure, studying epitope variability across clinical strains, and confirming molecular weight and surface expression (e.g., via Western Blot and Flow Cytometry).
Why Choose Us?
Choosing Creative Biolabs means partnering with over two decades of specialized expertise. Our M. pneumoniae P1 Specific Neutra™ Antibody Products offer a measurable advantage through:
- Unparalleled Specificity (Neutra™ Standard): Rigorous counter-screening against cross-reactive antigens guarantees minimal background signal and high diagnostic accuracy.
- High Functional Affinity: High binding affinity (in the nanomolar range) translates to greater assay sensitivity, critical for detecting low-titer samples.
- Validated Performance: Functionally validated for ELISA, Western Blot, IF, and IHC, ensuring immediate compatibility across multiple platforms.
- Optimized Stability and Consistency: Proprietary manufacturing ensures superior long-term stability and minimal lot-to-lot variation for multi-year projects.
FAQs
Q: How sensitive are these antibodies for detecting the P1 protein in environmental or low-concentration biological samples?
A: High functional sensitivity, enabling detection in the picogram-to-nanogram range due to optimized affinity, effective for identifying trace amounts in challenging samples.
Q: What is the risk of cross-reactivity with other common respiratory pathogens, such as Chlamydophila or other Mycoplasma species?
A: Minimizing non-specific binding is key. Antibodies are rigorously screened against a broad panel of related pathogens and host proteins to ensure high specificity, providing confidence in the P1-attributable signal.
Q: Are these reagents suitable for use in functional assays, such as blocking the bacteria's adhesion to host cells?
A: Yes, certain clones are specifically characterized to neutralize or inhibit P1 adhesin functional activity. Researchers interested in therapeutic/preventative applications should inquire about our designated neutralizing clones.
Q: What is the recommended storage and handling procedure to maintain the long-term activity and stability of the antibody product?
A: For maximum longevity, store at -20℃ to -80℃ as specified on the datasheet. Avoid repeated freeze-thaw cycles by aliquoting upon receipt to ensure reagent integrity.
Q: How do these high-specificity monoclonal antibodies compare to traditional polyclonal antibody preparations for diagnostic applications?
A: Monoclonal reagents provide superior consistency, reproducibility, and minimal lot-to-lot variability compared to polyclonal antibodies. Their single-epitope specificity is essential for standardizing diagnostic assays and developing robust, commercial-ready kits.
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REFERENCE
- Jiang, Zhulin et al. "Mycoplasma pneumoniae Infections: Pathogenesis and Vaccine Development." Pathogens (Basel, Switzerland) vol. 10,2 119. 25 Jan. 2021, Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3389/fmicb.2016.00513
