esxB Specific Neutra™ Antibody Products
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Are you currently facing challenges in accurately characterizing Mycobacterium tuberculosis (Mtb) virulence factors, struggling with the development of sensitive, next-generation TB diagnostic assays, or seeking reliable reagents to study host-pathogen interactions in high-containment environments? Our esxB Specific Neutra™ Antibody Products help you accelerate drug discovery and immunological research by providing high-affinity, rigorously validated detection tools for a critical Mtb secretion component, enabling precise quantification and cellular localization studies through advanced immunoassay techniques.
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Introduction of esxB
Mycobacterium tuberculosis (Mtb), the cause of tuberculosis (TB), uses the Type VII Secretion System 1 (ESX-1) to survive and replicate inside host cells, exporting key proteins for survival. esxB, a founding member of the WXG100 family, is a major ESX-1 substrate. It forms a stable 1:1 heterodimer with its partner, esxA (ESAT-6), and this complex is the primary virulence determinant of the ESX-1 locus. The esxA-esxB complex is crucial for immune evasion, mediating Mtb's translocation from the phagolysosome into the host cell's cytosol. esxB also activates human neutrophils and its deletion, as seen in the Mycobacterium bovis BCG vaccine strain, significantly impairs virulence, emphasizing its critical role.
Fig.1 Model of the MtbESX-1 core membrane complex.1
Antibodies Against esxB
Due to its critical role in virulence and strong immunogenicity, esxB is a primary target in Mtb research. Anti-esxB antibodies are indispensable molecular probes for both basic research and diagnostic tool development.
These high-specificity antibodies are crucial for dissecting ESX-1 driven pathology, tracking esxB's localization from secretion into the culture filtrate to its presence inside host cells following phagosomal rupture. Applications include:
- Western Blotting (WB): For verifying esxB expression, purification, and presence in Mtb strains or infected cellular extracts.
- Enzyme-Linked Immunosorbent Assay (ELISA): To quantify esxB in clinical or environmental samples (sandwich/indirect ELISA), aiding rapid TB diagnostic test development.
- Immunohistochemistry (IHC) and Immunofluorescence (IF): Essential for visualizing esxB within host cells/tissues, mapping its distribution, tracking translocation (phagosome to cytosol), and studying interactions with immune machinery (e.g., T-cells).
- Flow Cytometry: To assess esxB binding to host immune cells (monocytes/macrophages), supporting the study of cell signaling.
Targeting esxB allows researchers to move beyond gene-level analysis to functional protein studies, offering deeper insights into the host-pathogen interface.
Why Choose Us?
Creative Biolabs' commitment to quality ensures our esxB Specific Neutra™ Antibodies meet stringent research demands. We provide unparalleled value via key differentiators: Exceptional Specificity (minimal cross-reactivity for high-confidence data), Rigorous Validation (guarantees lot consistency across multiple assays), and flexible Custom Formats. Our proven performance in leading institutions is supported by Published Data, and we offer Dedicated Expert Support with over 20 years of experience.
FAQs
Q: Is this antibody suitable for detecting the protein in complex biological matrices?
A: Yes, due to its high specificity. However, for this low-abundance secreted protein, optimal detection in complex samples (serum, media) often requires sample concentration protocols like immunoprecipitation or ultrafiltration.
Q: Given the protein forms a heterodimer, will this antibody recognize the monomeric form, the heterodimer, or both?
A: Our immunization strategy uses the full-length protein, generating antibodies that typically recognize both the active heterodimer and the monomeric form (targeting both linear and conformational epitopes).
Q: What is the optimal Western Blotting protocol for this small protein?
A: For this ~10 kDa protein, we recommend using Tris-Tricine gels for optimal separation, a small-pore PVDF membrane for efficient transfer, and optimized blocking/rigorous washing to maximize the signal-to-noise ratio.
Q: How does this antibody aid in distinguishing virulent strains from attenuated vaccine strains?
A: Since the encoding gene is typically deleted in attenuated vaccine strains, this antibody is a reliable marker to distinguish virulent, ESX-1 positive strains from non-virulent or vaccine-related counterparts.
Q: Are there any known cross-reactivities with common laboratory bacteria or host proteins?
A: While the WXG100 family is conserved, our screening minimizes cross-reactivity. Researchers should always run negative controls (uninfected cells or non-expressing strains) to empirically verify specificity.
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REFERENCE
- Ly, Angel, and Jun Liu. "Mycobacterial Virulence Factors: Surface-Exposed Lipids and Secreted Proteins." International journal of molecular sciences vol. 21,11 3985. 2 Jun. 2020, Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/ijms21113985
