proBNP Specific Neutra™ Antibody Products
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Are you currently facing challenges in early and accurate diagnosis of heart failure, difficulty in standardizing immunoassay development, or the need for highly sensitive prognostic biomarkers? Our Creative Biolabs Pro-Brain Natriuretic Peptide Specific Neutra™ Antibody Products help you accelerate diagnostic kit development, obtain high-quality immunoassay components, and standardize patient stratification through high-affinity, specific antibody engineering and rigorous validation protocols.
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Introduction of proBNP
Pro-Brain Natriuretic Peptide (proBNP) is a 108-amino acid prohormone predominantly synthesized by ventricular cardiomyocytes in response to increased hemodynamic stress, particularly ventricular volume expansion and wall tension. proBNP acts as a critical signal in the body's attempt to maintain fluid balance and reduce cardiac strain. Upon release into the circulation, the prohormone is rapidly cleaved by convertases (such as furin) into two fragments in equimolar amounts: the biologically active C-terminal peptide, Brain Natriuretic Peptide (BNP-32), and the biologically inactive N-terminal fragment, NT-proBNP.
NT-proBNP has emerged as the superior clinical biomarker for heart failure due to its extended plasma half-life of approximately 60-120 minutes (compared to the short 20-minute half-life of active BNP) and its higher circulating concentrations. Its stability makes it less susceptible to pre-analytical errors and more suitable for reliable measurement in diverse laboratory settings. Elevated NT-proBNP levels are highly correlated with the severity of ventricular dysfunction and are universally accepted for the screening, diagnosis, and prognosis of acute and chronic heart failure, as summarized in widely cited literature. Measurement of NT-proBNP is foundational in understanding the natriuretic peptide signaling pathway, which operates by activating the natriuretic peptide receptor A (NPRA), leading to downstream effects of diuresis, natriuresis, and systemic vasodilation.
Fig.1 Physiology of BNP and NT-proBNP production.1
Antibodies Against proBNP
The development of high-performance immunoassays relies entirely on the quality and specificity of the anti-proBNP and anti-NT-proBNP antibodies used. Given that NT-proBNP is the preferred clinical target, antibodies are specifically generated to bind to different, non-overlapping epitopes on this fragment to ensure robust Sandwich assay performance.
Our Neutra™ Antibody Products are optimized for binding the most stable regions of the NT-proBNP molecule, providing exceptional resilience across varied sample matrices (serum, plasma). Applications are diverse, covering the full spectrum of cardiovascular research and diagnostic development:
- Clinical Diagnostic Assays: Serving as the key components in quantitative immunodiagnostic kits (e.g., ELISA, POCT devices) for heart failure.
- Prognostic & Monitoring Studies: Used in research to accurately track changes in circulating NT-proBNP levels following pharmacological or interventional therapies.
- Immunohistochemistry (IHC) & Western Blotting: Used for the localization and detection of proBNP and NT-proBNP protein expression within myocardial tissue samples, aiding basic research into the underlying pathology of cardiac remodeling.
Why Choose Us?
Choosing Creative Biolabs means partnering with a team that has over two decades of specialized expertise in high-sensitivity biomarker immunoassay development. Our focus on proBNP antibody engineering translates directly into superior product quality and a significant competitive advantage for your final diagnostic product.
Key Advantages of Creative Biolabs' Neutra™ Antibody Products:
- Verified High Affinity & Specificity: Rigorously tested to ensure exceptional binding affinity to NT-proBNP and minimal cross-reactivity with the active BNP fragment, leading to highly accurate assay results (Published Data).
- Validated Matched Pairs: We supply pre-validated capture and detection antibody pairs specifically optimized for Sandwich immunoassays, dramatically accelerating your development timeline.
- Industry-Leading Consistency: Utilizing standardized proprietary purification methods, we guarantee exceptional lot-to-lot consistency, crucial for regulatory approval and maintaining commercial stability.
FAQs
Q: Why is the N-terminal fragment often the preferred target over the active hormone for diagnostic testing?
A: NT-proBNP is preferred because it is inactive and has a significantly longer half-life than active BNP. This stability provides a more reliable, reproducible result that accurately reflects the patient's long-term cardiac wall stress status.
Q: What are the critical considerations when selecting antibody pairs for a quantitative immunoassay?
A: For quantitative assays, selecting two antibodies that bind distinct, non-overlapping epitopes is crucial. This non-interference ensures a robust "sandwich" configuration, maximizing sensitivity and minimizing steric hindrance.
Q: Can these reagents be adapted for use in point-of-care (POCT) diagnostic devices?
A: Yes. The high stability, excellent specificity, and strong signal generation make these reagents ideal for integration into rapid POCT formats, such as lateral flow devices. Successful use requires optimizing concentration and conjugation protocols for the device matrix.
Q: How can I ensure the accuracy of the assay when dealing with varied clinical samples (e.g., serum vs. plasma)?
A: To ensure accuracy across matrices (e.g., serum vs. plasma), select reagents robust against interferences. Thorough validation with comprehensive clinical samples, appropriate dilution, and buffer optimization is necessary for reliable, consistent assay performance.
Q: What is the main scientific advantage of using highly characterized, specific antibodies compared to lower-cost alternatives?
A: The main advantage is reliability and precision. Highly characterized antibodies minimize false results by preventing cross-reactivity or interference from circulating factors. This scientific rigor yields higher data confidence and superior clinical utility for diagnostic products, ultimately saving time and resources.
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REFERENCE
- Ludwikowska, Kamila Maria et al. "Clinical Significance of B-Type Natriuretic Peptide and N-Terminal Pro-B-Type Natriuretic Peptide in Pediatric Patients: Insights into Their Utility in the Presence or Absence of Pre-Existing Heart Conditions." International journal of molecular sciences vol. 25,16 8781. 12 Aug. 2024, Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/ijms25168781
