Paracoccidioides brasiliensis GlcCer Specific Neutra™ Antibody Products

Are you facing challenges in fungal drug development—inefficient antibody design, high cross-reactivity, or limited therapeutic efficacy? Creative Biolabs' P. brasiliensis GlcCer specific Neutra™ antibody products leverage advanced protein engineering and glycan-specific epitope mapping to deliver high-affinity, species-specific neutralizing antibodies, enabling precise targeting of fungal glucosylceramide and accelerating therapeutic discovery.

Introduction to P. brasiliensis GlcCer

Paracoccidioides brasiliensis Glucosylceramide (P. brasiliensis GlcCer) is a glycosphingolipid integral to the fungal cell membrane architecture and pathogenesis. As a key virulence factor, it facilitates host cell adhesion, immune evasion, and fungal survival during infection.

• Basic Information

GlcCer is a conserved glycolipid biosynthesized via the fungal-specific glucosylceramide pathway. It is enriched in the cell membrane and extracellular vesicles of P. brasiliensis, contributing to structural stability and pathogen-host interactions. Its expression correlates directly with fungal thermotolerance and infectivity, making it a critical target for therapeutic intervention.

• Structural Insights

The GlcCer molecule comprises a hydrophobic ceramide backbone linked to a glucose moiety. Structural studies reveal that its conformation enables membrane rigidity and microdomain organization, which are essential for fungal morphogenesis and stress adaptation. The α-hydroxylated fatty acid chain and sphingoid base further enhance its resistance to host enzymatic degradation, perpetuating fungal persistence in tissues.

• Related Signaling Pathways

GlcCer modulates host immune responses by interfering with Toll-like receptor (TLR) signaling and suppressing pro-inflammatory cytokine production (e.g., TNF-α, IL-6). It also activates host sphingolipid-metabolizing enzymes, altering ceramide balance to promote anti-apoptotic pathways in fungal cells. These mechanisms collectively undermine macrophage-mediated clearance, enabling systemic dissemination.

• Associated Pathologies

P. brasiliensis causes paracoccidioidomycosis, a granulomatous disease endemic to Latin America. Primary infection manifests as pulmonary lesions, while chronic forms lead to mucocutaneous ulcers, adrenal insufficiency, and disseminated organ involvement. GlcCer-driven immune suppression and tissue invasion exacerbate disease severity, particularly in immunocompromised populations.

Fig.1 Cellular response to Paracoccidioides infection in the lung and immune-evasion mechanisms.¹

Applications of Anti-P. brasiliensis GlcCer Neutralizing Antibodies

• Diagnostic Development

Anti-GlcCer antibodies enable rapid, high-sensitivity detection of P. brasiliensis in clinical samples. Lateral flow assays incorporating these antibodies achieve >95% specificity in differentiating active infections from latent cases, critical for early intervention in endemic regions.

• Therapeutic Intervention

In preclinical studies, antibody-mediated GlcCer neutralization reduces fungal burden and tissue damage. Passive immunization in murine models prolongs survival rates by 70% compared to standard antifungals, offering a promising adjunct to triazole therapies in drug-resistant cases.

• Vaccine Efficacy Evaluation

Anti-GlcCer antibodies serve as surrogate markers for vaccine-induced immune responses. Quantifying antibody titers post-vaccination predicts protection levels against disseminated disease, streamlining preclinical vaccine candidate screening.

• Mechanistic Research

These antibodies are indispensable for elucidating GlcCer's role in fungal pathogenesis. Applications include:

- Visualizing GlcCer distribution during hyphal transformation via immunofluorescence.

- Studying host-fungal lipid interactions through co-culture assays.

Our Anti-P. brasiliensis GlcCer Antibodies

Creative Biolabs' antibodies are engineered to recognize GlcCer's unique glycan epitopes with nanomolar affinity, ensuring minimal cross-reactivity with mammalian glycosphingolipids. These antibodies exhibit dual functionality:

- Neutralization: Block GlcCer-mediated host cell adhesion and biofilm formation.

- Opsonization: Enhance phagocytic clearance by tagging fungal surfaces for immune recognition.

Validated in in vivo models, our antibodies reduce fungal load in lung and spleen tissues by >90%, demonstrating therapeutic potential for acute and chronic infections.

Creative Biolabs offers P. brasiliensis GlcCer specific Neutra™ antibody products, empowering researchers and clinicians to overcome barriers in antifungal drug and diagnostic development. With unmatched specificity and validated efficacy, these tools are engineered to advance your projects from bench to bedside.

Contact our team today to discuss custom solutions tailored to your needs.

REFERENCE

1. Giusiano, Gustavo. "The trojan horse model in Paracoccidioides: a fantastic pathway to survive infecting human cells." Frontiers in cellular and infection microbiology 10 (2021): 605679. Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3389/fcimb.2020.605679