PBP2a Specific Neutra™ Antibody Products
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Are you currently facing long drug development cycles for new anti-MRSA compounds, or difficulties in accurately detecting Methicillin-Resistant Staphylococcus aureus (MRSA) resistance mechanisms? Our PBP2a Specific Neutra™ Antibody Products help you accelerate drug discovery and obtain high-quality, reliable diagnostic data through advanced immunoassay tools and high-specificity antibody engineering.
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Introduction of PBP2a
Penicillin-binding proteins (PBPs) are bacterial enzymes that are essential for the final stages of peptidoglycan synthesis, the primary component of the cell wall. They are the molecular targets of β-lactam antibiotics, which inhibit their function by acting as substrate mimics.
PBP2a's Structure and Function in Resistance:
PBP2a (or PBP2'), encoded by the mecA gene, is the primary mediator of β-lactam resistance in MRSA. This protein is structurally unique due to its low affinity for β-lactams compared to native S. aureus PBPs. As a 76 kDa transpeptidase anchored to the cytoplasmic membrane, PBP2a continues cell wall synthesis (transpeptidation) even when native PBPs are inactivated. Its large, globular transpeptidase domain ensures bacterial survival despite high antibiotic concentrations.
Clinical and Biological Significance:
The acquisition and expression of PBP2a are central to the global public health crisis posed by MRSA. Research tools, such as the PBP2a Neutra™ Antibody, are critical for understanding PBP2a's structural dynamics, regulatory pathways, and cellular localization. This knowledge is paramount for developing next-generation antibiotics that can bypass or inhibit its function, and for elucidating the precise mechanism by which PBP2a maintains its resistance.
Fig.1 Structure of PBP2a.1
Antibodies Against PBP2a
Highly specific PBP2a antibodies revolutionize MRSA detection and study. They selectively target PBP2a's unique epitope, distinguishing it from native, β-lactam-sensitive PBPs.
Application Versatility in Research and Diagnostics:
- IHC/IF: Visualizes PBP2a expression and distribution in cells or tissue, aiding resistance regulation study.
- Western Blotting: Provides precise size and presence confirmation of PBP2a protein extracts, essential for induction or inhibition studies.
- Rapid Diagnostics: High affinity enables fast, accurate point-of-care devices that detect PBP2a in patient samples, supporting timely treatment decisions over culture methods.
- ELISA/IP: Utilized for quantitative measurement (ELISA) and isolation (IP) of PBP2a for structural or functional analysis.
Creative Biolabs' PBP2a Neutra™ Antibodies ensure superior specificity and reliable signal across all major immunoassay platforms, significantly improving data fidelity in anti-MRSA research and diagnostics.
Why Choose Us?
Choosing Creative Biolabs means partnering with a specialist. Our PBP2a antibodies deliver unparalleled consistency and performance, accelerating your anti-infective solutions.
Key Advantages:
- Ultimate Specificity: Rigorous testing guarantees zero cross-reactivity against sensitive strains.
- High Sensitivity: Protocols detect low PBP2a levels, validated for robust ELISA, WB, and IHC.
- Reliable Consistency: Stringent QC minimizes lot variation.
- Expert Support: Years of specialist consultation available.
FAQs
Q: Why is it important to use an antibody that specifically targets PBP2a rather than a generic PBP marker when diagnosing methicillin resistance?
A: Methicillin resistance requires PBP2a, an enzyme with dramatically reduced affinity for β-lactams. Since all S. aureus strains have native PBPs, using a specific antibody detects the resistance mechanism itself, leading to precise, clinically relevant results without false positives from sensitive strains.
Q: Can this type of antibody be used to monitor the effectiveness of novel β-lactamase inhibitors in a cellular context?
A: Absolutely. These antibodies are ideal for mechanistic studies. By performing Immunofluorescence (IF) or Western Blotting (WB) on treated bacterial cells, you can monitor PBP2a expression and localization. A change in the PBP2a signal serves as a key visual or quantitative indicator of the compound's impact on the resistance mechanism.
Q: What is the recommended starting application for assessing PBP2a expression in purified bacterial cultures?
A: Western Blotting is the gold standard for initial verification and quantification from purified lysates. It confirms the correct ~76 kDa molecular weight and expression levels before moving to more complex spatial analyses like IHC or IF.
Q: Are there any known cross-reactivities or precautions I should be aware of when using PBP2a-specific antibodies?
A: High-quality PBP2a antibodies are engineered to avoid cross-reactivity with the native, β-lactam-sensitive PBPs. The primary precaution is proper sample preparation: since PBP2a is membrane-anchored, efficient cell lysis and membrane solubilization are critical for successful detection in applications like Western Blotting or ELISA.
Q: How does an antibody-based rapid test for PBP2a compare to DNA-based PCR methods for MRSA detection in terms of speed and information provided?
A: Both are fast but provide different information. PCR detects the mecA gene (genetic potential for resistance). An antibody-based test detects the PBP2a protein itself, confirming its expression and functional presence. The antibody test is often simpler and faster at the point of care, directly identifying the resistance phenotype for immediate clinical decisions.
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REFERENCE
- Aribisala, Jamiu Olaseni, and Saheed Sabiu. "Cheminformatics Identification of Phenolics as Modulators of Penicillin-Binding Protein 2a of Staphylococcus aureus: A Structure-Activity-Relationship-Based Study." Pharmaceutics vol. 14,9 1818. 29 Aug. 2022, Distributed under Open Access license CC BY 4.0, without modification. https://doi.org/10.3390/pharmaceutics14091818
