PEDV Specific Neutra™ Antibody Products

Creative Biolabs is a reliable partner for sourcing premium PEDV antibody solutions. Our approach integrates advanced technological platforms with deep-seated expertise to provide products that consistently satisfy stringent research demands.

• Deliver comprehensive, integrated solutions to address multifaceted challenges, ensuring our products meet the highest quality standards.
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Introduction of PEDV

Porcine epidemic diarrhea virus (PEDV), a member of the Alphacoronavirus genus (family Coronaviridae), exhibits strict enterotropism for porcine intestinal enterocytes, precipitating fulminant enteritis characterized by profuse diarrhea, dehydration, and mortality rates approaching 100% in neonates within 120 hours post-infection. Post-weaning and adult swine display transient clinical manifestations—notably anorexia and progressive cachexia—though fatalities remain infrequent. Notably, PEDV demonstrates strict species specificity, posing no zoonotic risk or foodborne transmission threat.

The ~28 kb positive-sense RNA genome encodes four structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N), in addition to accessory nonstructural polypeptides that modulate host-pathogen interactions. The homotrimeric S glycoprotein, a class I fusion protein, orchestrates viral ingress through high-affinity engagement (K_D ≈ 4.2 nM) of porcine aminopeptidase N (pAPN) receptors, while its receptor-binding domain (RBD) governs strain-specific pathobiology. Mechanistically, pH-dependent conformational rearrangements in the S1 subunit facilitate endosomal membrane fusion, a process potentiated by trypsin-like protease cleavage. This structural plasticity underpins critical virological adaptations: evasion of neutralizing antibodies through glycan shielding of antigenic sites, attenuation of virulence via truncations in the S1 N-terminal domain, and tissue tropism modulation through sialic acid-binding activity.^{1, 4}

Fig. 1 Schematic illustration of PEDV infection in host cell.^2,4

Although the functional receptor for PEDV was discovered over four decades ago, it remains unidentified. While APN is thought to be a binding receptor for PEDV, recent investigations indicate that APN alone induces PEDV infection, and PEDV infection can still occur in APN knockout pigs. The entry of PEDV into cells involves clathrin, caveolin, occludin, and transferrin receptor 1. However, these factors still fail to fully elucidate the molecular mechanism behind PEDV cell tropism, the extremely low efficiency of in vitro PEDV isolation, and the subsequent loss of infectivity and partial immunogenicity observed in adapted strains during serial cell culture passage. This not only impedes fundamental PEDV research but also significantly hampers the development of PEDV vaccines. Furthermore, despite its high pathogenicity in pigs, PEDV exhibits insensitivity to certain porcine cell lines, which somewhat limits studies on PEDV virulence and vaccine development.

Antibodies against PEDV

Porcine epidemic diarrhea manifests as acute emesis and diarrheal morbidity in immunologically naive swine populations, with suckling neonates (<14 days) exhibiting mortality rates exceeding 80%. Non-infected offspring lack transplacental immunity, necessitating postparturient acquisition of lactogenic immunoglobulins (IgG/IgA) via colostral transfer during the critical 24-hour neonatal window. This underscores the imperative for pre-farrowing vaccination protocols in dams to boost secretory antibody titers in mammary secretions.

Colostral IgG constitutes >60% of humoral immune factors, though its concentration plummets in mature milk due to mammary epithelial tight junction closure. While IgA represents <15% of colostral antibodies, its dimeric structure confers protease resistance and superior neutralization kinetics against enteric pathogens compared to IgG/IgM—attributes critical for mucosal immunity in the gastrointestinal milieu.

Published Data

Fig. 2 Functional analysis of the mAbs by IFA and FCA.^{3, 4}

The HEK293F mammalian system was utilized to generate glycosylated S1 antigens through transient transfection. Five murine-derived monoclonal antibodies (mAbs) targeting PEDV-S1 were generated via hybridoma screening, with binding kinetics quantified through ELISA, immunofluorescence (IFA), and flow cytometric assays (FCA). Discrepancies emerged between clones 6-B5 and 8-G2 across fixation-dependent (IFA) and live-cell (FCA) platforms, suggesting formaldehyde cross-linking alters epitope accessibility. Specifically, 8-G2 and 6-B5 exhibited fixation-enhanced binding kinetics, demonstrating 3.7-fold higher affinity for aldehyde-treated virions compared to native conformations. While 2-C9 and 8-G2 shared comparable half-maximal effective concentrations, their FCA signal intensities diverged 4.5-fold, likely reflecting antigenic divergence between recombinant S1 immunogens and native viral spike complexes. This work yielded a stable S1 antigenic scaffold and five neutralizing mAbs, enabling high-sensitivity diagnostics and mechanistic investigations of coronavirus entry mechanisms.

Why Choose Us?

Creative Biolabs is a trusted provider of high-quality antibodies, including those targeting IAV M2e. We offer several key advantages:

• Specificity and Sensitivity

Our antibodies are generated using carefully designed antigens and undergo rigorous screening to ensure exceptional specificity and sensitivity, minimizing off-target binding and maximizing signal-to-noise ratios.

• Customization Options

We offer a range of antibody formats and modifications to meet your specific research needs. The options include a variety of isotypes, which can influence effector functions; conjugates (e.g., HRP, FITC) for diverse detection methods; and purification levels, tailored to the required purity for your application.

• Expert Support

Our team of experienced scientists provides comprehensive technical support, assisting you with experimental design, antibody selection, and troubleshooting.

• Reliable Performance

Our antibodies are manufactured under strict quality control standards, ensuring consistent performance and reproducibility in your experiments.

For more information about Creative Biolabs' PEDV antibodies and services, please contact us.

REFERENCES

1. Li, Zhiwei, et al. "Molecular mechanism of porcine epidemic diarrhea virus cell tropism." Mbio 13.2 (2022): e03739-21.
2. Zhang, Yuanzhu, et al. "Porcine epidemic diarrhea virus: An updated overview of virus epidemiology, virulence variation patterns and virus–host interactions." Viruses 14.11 (2022): 2434.
3. Lang, Qiaoli, et al. "High-affinity monoclonal antibodies against the porcine epidemic diarrhea virus S1 protein." BMC Veterinary Research 20.1 (2024): 239.
4. Distributed under Open Access license CC BY 4.0, without modification.